Quantitative PCR arrays are the most reliable and accurate tool for parallel quantitative analysis of gene expression relevant to a pathway or a disease state, with the sensitivity and specificity of a real-time PCR (qPCR) as well as the multi-gene profiling capability of a microarray. PCR arrays are available in both 96-well, 384-well and 1536-well formats for detecting the expression of 84 up to over 1000 target genes plus multiple reference genes.
The two most used PCR arrays are based on SYBR® Green or TaqMan® qPCR assays. SYBR® Green qPCR is widely used due to its simple assay design and its relatively low assay setup and running costs. The SYBR® Green dye intercalates into double stranded DNA to detect the amplification of the target gene specifically initiated by gene-specific primers. However, the dye is no specific for a DNA sequence, so it can detect all amplified double-stranded DNA, including non-specific reaction products. Unlike SYBR® Green, only specific PCR product can produce a fluorescent signal in TaqMan® qPCR, because the method uses a fluorogenic single-stranded oligonucleotide probe that binds only to the DNA sequence between the two PCR primers, which significantly increases the specificity of the detection.
The workflow for using PCR arrays is simple. The PCR template (cDNA) is combined with a ready-to-use qPCR master mix, and equal aliquots are added to each well of the same plate already containing gene-specific primer sets and probe. Then, the qPCR cycling program is performed and threshold cycle (Ct) values for all the genes on each PCR array are calculated.
Amerigo Scientific offers high-quality qPCR arrays of different types, species, and formats for the study of pathological pathways (neurological diseases, autoimmune diseases, mental disorders, cancer, etc.), pharmacological pathways, and cellular signaling pathways in a variety of applications, such as gene expression profiling, drug development, biomarker signature identification, and validation of microarray results.