Proteins are complex structures with large surface areas that contain a variety of stereogenic centers and different binding sites, which gives many proteins the potential to discriminate between the separate forms of a chiral compound. Various types of proteins such as enzymes, serum transporters, and glycoproteins, can be immobilized on supports and used as chiral stationary phases (CSPs) for high performance liquid chromatography (HPLC). The separation of chiral compounds is achieved based on differences in the binding strength and the amount of binding sites for enantiomers with the proteins. The advantages of using protein-based CSPs include their tendency to be able to act on a wide range of chiral compounds, their ability to be used in aqueous mobile phases, and their ability to directly analyze most chiral compounds without derivatization.
CHIRALPAK® Protein Chiral Stationary Phases
CHIRALPAK® AGP, HSA, and CBH stationary phases are 5-µm spherical silica particles immobilized with protein chiral selectors, namely α1-acid glycoprotein, human serum albumin, cellobiohydrolase (stable enzyme), respectively. The CHIRALPAK® protein CSPs can be operated in reversed-phase mode using buffers with low organic modifier content and at moderate pH. Their enantioselectivity can be easily controlled or improved by changing the mobile phase composition including pH, buffer, and type and concentration of organic modifiers.
CHIRALPAK® Protein-based Chiral HPLC Columns
Amerigo Scientific provides chiral HPLC columns based on immobilized protein CSPs for separation and characterization of chiral compounds across a wide range of applications such as drug discovery, quality assurance, environmental monitoring, and drug-protein binding studies.
Applications
CHIRALPAK® AGP columns have extremely wide applicability and can be used to separate various chiral compounds including amine (primary, secondary, and tertiary), acids (strong and weak), and nonprotolytes (amides, esters, alchohols, sulfoxides, etc.).
The enantioselectivity of the CHIRALPAK® CBH selector is complementary to that of the CHIRALPAK® AGP selector. CHIRALPAK® CBH columns are suitable for the separation of containing one or more basic nitrogen together with one or more hydrogen accepting or hydrogen donating groups (alcohol, phenol, carbonyl, amide, ether, sulphoxide, ester, etc.).
CHIRALPAK® HAS columns are especially suited for the separation of weak and strong acids, zwitterionic and nonprotolytic compounds.
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