Gel4Cell® and PolyInks® Bioinks

Products List

Bioinks are 3D bioprinting materials composed of cells, hydrogel and bioactive molecules used to create engineered and artificial biological tissues. Bioink is considered to be one of the most advanced tools in tissue engineering and regenerative medicine. As a material for the construction of a specific biological tissue or organ, bioink must have physical and mechanical properties, biofunctionality, biocompatibility, etc. The ultimate goal of tissue engineering and regenerative medicine is to replace damaged tissues and organs with new and functional tissues and organs composed of various cells. Bioprinting technology that can achieve this goal is attracting close attention from academia and industry. As a result, the field of artificial organs using 3D bioprinting is growing rapidly.

Amerigo Scientific offers high-function bioinks that composed of bioactive factors or human-derived collagen / extracellular matrices and hydrogels, providing the optimal environment for living cells to create artificial organs.

Gel4Cell® Hydrogel Bioinks

Gel4Cell® is a gelatin-based hydrogel bioink for 3D bioprinting with improved biocompatibility and structural safety. This bioink can also be used for many other studies that require 3D culture and structure formation, including cell biology, biochemistry, pharmacology, physiology, biomaterials sciences, bioengineering, and drug testing and development. In addition, we provide bioinks based on Gel4Cell®, which contain optimized active biomolecular mimicking peptides conjugated gelatin-based hydrogel complex, such as vascular endothelial growth factor (VEGF), transforming growth factor (TGF), or bone morphogenetic protein-2 (BMP-2).

These bioink kits include optimally formulated gelatin-based hydrogel complex packaged in syringes for easy dispensing. Gel-linker for reinforcing mechanical stability is also included. Transparent hydrogels are formed when contents are properly mixed and exposed to UV light.

Product Name	Description	Applications
Gel4Cell®	Gelatin hydrogel bioink	3D bioprinting
Gel4Cell®-TGF	Hydrogel bioink with TGF-β1 peptide	Cartilage regeneration
Gel4Cell®-BMP	Hydrogel bioink with BMP-2 peptide	Bone regeneration
Gel4Cell®-VEGF	Hydrogel bioink with VEGF peptide	Skin and blood vessel regeneration
Gel4Tissue®	Hydrogel bioink with SIS-ECM	Artificial organs and tissues regeneration
Col4Cell	Collagen hydrogel bioink	Tissue engineering, 3D bioprinting, and drug delivery systems

PolyInks® Polymer Bioinks

Poly-ε-caprolactone (PCL) and poly(L-lactide) (PLA) are biodegradable polyesters with melting temperature of around 60 °C and 180 °C, respectively. They have been widely used in a variety of biomedical applications. Both PCL and PLA were approved for use in multiple clinical applications, including drug delivery devices, sutures, and adhesion barriers. PCL and PLA are degraded by hydrolysis of their ester linkages in the human body, but the degradation rate of PCL is much slower than that of poly-lactide or -glycolide. PCL and PLA are often used in 3D bioprinting due to their low melting temperature, easy delivery through a fine nozzle, and strong adhesive properties between the deposition layers. Moreover, they readily form and maintain 3D structures with durable structural integrity.

Amerigo Scientific offers Polyinks®-PLA powder and PolyInks®-PCL filament that are research grade polymers. These polymer products have been shown to be easily dispersed and build 3D structures using a commercially available 3D printer. For applications involving cells, Polyinks® products are best used in combination with gelatin-based Gel4Cell® products.

Product Name	Description	Applications
PolyInks®-PLA	L-lactide synthetic polymer	3D bioprinting
PolyInks®-PCL	Caprolactone synthetic polymer	Tissue engineering, 3D bioprinting, and drug delivery systems
PolyInks®-PLCL-W	Synthetic polymer with the mixture of PLA and PCL	Tissue engineering, 3D bioprinting, and drug delivery systems
PolyInks®-PLCL-Y
PolyInks®-PLCL-M

Applications

Specific cells and culture agents are involved in creating artificial organs with specific shapes and properties. Our bioinks consist of gelatin, hyaluronic acid, and glycerol. They are human body-friendly and have a wide range of applications.

All Gel4Cell®products can be used to culture mesenchymal stem cells (MSCs) and induced pluripotent stem cells (iPSCs). In addition, Gel4Cell®-TGF can be applied to study cartilage regeneration by using TGF-β1 peptide as a cartilage regeneration growth factor. Gel4Cell®-BMP containing BMP-2 peptide can be used for bone regeneration research. Gel4Cell®-VEGF can be used for skin and blood vessel regeneration research.

▪ Cell Culture

Product Name	Normal Cells	MSCs	iPSCs	Osteogenesis	Chondrogenesis	Angiogenesis	Scaffold
Gel4Cell®	✔	✔	✔				✔
Gel4Cell®-BMP		✔	✔	✔			✔
Gel4Cell®-VEGF		✔	✔		✔		✔
Gel4Cell®-TGF		✔	✔			✔	✔
PolyInks®-PCL&PLA							✔
PolyInks®-PLCL W, M, Y							✔
Gel4Tissue®	✔	✔	✔				✔
Col4Cell	✔	✔	✔				✔

▪ Organoid Culture

Product Name	Skin	Bone	Cartilage	Vascular	Organoid	Spheroid
Gel4Cell®	✔	✔	✔	✔	✔	✔
Gel4Cell®-BMP		✔
Gel4Cell®-VEGF	✔			✔
Gel4Cell®-TGF			✔
PolyInks®-PCL&PLA	✔	✔	✔	✔
PolyInks®-PLCL W, M, Y	✔	✔	✔	✔
Gel4Tissue®	✔	✔	✔	✔	✔	✔
Col4Cell	✔	✔	✔	✔	✔	✔

Printability and Stability

Gel4Cell® bioink, Gel4Tissue® bioink, Col4Cell bioink all maintain their physical properties stably after printing or over a period of time.

Operational Process

Cell Viability/Proliferation

The Gel4Cell® bioink showed excellent physical properties as well as high cell viability.

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