Lipotoxicity is caused by intracellular lipid accumulation and is indicative of mitochondrial disfunction. Lipotoxicity accelerates the degenerative process of cellular senescence, influencing cancer development.
The Lipi-series kits can clearly identify lipid droplets with an extremely low level of background fluorescence and specificity better than that of BODIPY 493/503 or Nile Red.
We observed lipid droplet generation in young and aged mice using mouse liver adipose tissue (frozen sections).
Lipi-series kits are available in four colors (blue, green, red, and deep-red) and can be used for multiple stain analysis as shown in the following papers.
Sample | Multiple Staining | Reference |
---|---|---|
Brown Adipocytes | Co-stained with MitoBright Green (Mitochondria), Hoechst 33342 (Nuclear) and Lipi-Red | Masako, O. et al., "Exogenous Cytokine-Free Differentiation of Human Pluripotent Stem Cells into Classical Brown Adipocytes", Cells., 2019, 8(4), 373. |
hMGEC | Co-stained with tandem RFP-GFP-tagged LC3B (autophagosome /autophagolysosome) and Lipi-Blue | Kim, S. et al., "Eicosapentaenoic acid (EPA) activates PPAR? signaling leading to cell cycle exit, lipid accumulation, and autophagy in human meibomian gland epithelial cells (hMGEC)", The Ocular Surface, 2020, 18(3), 427-437. |
AdMSCs | Co-stained with UCP1 antibody (Alexa Fluor 488), DAPI (Nuclear) and Lipi-Red | Yukimasa, T. et al., "Transcriptome analysis reveals brown adipogenic reprogramming in chemical compound-induced brown adipocytes converted from human dermal fibroblasts", Scientific Reports, 2021, 11, 5061. |
Function | CAT. No. | Product Name | Size |
---|---|---|---|
Lipid droplet imaging | LD01 | Lipi-Blue | 10 nmol |
LD02 | Lipi-Green | 10 nmol | |
LD03 | Lipi-Red | 100 nmol | |
LD04 | Lipi-Deep Red | 10 nmol | |
Lipid droplet quantification (plate reader, FCM) | LD05 | Lipid Droplet Assay Kit-Blue | 1 set |
LD06 | Lipid Droplet Assay Kit-Deep Red | 1 set |
Note: If you don't receive our verification email, do the following: