Cell-free DNA (cfDNA) is DNA fragment that freely circulates in the human bloodstream. In healthy individuals, the death of normal hematopoietic cells is the main contributor of plasma cfDNA. In cancer patients, the presence of circulating tumor DNA (ctDNA) fragments from tumor cells in plasma is a cancer biomarker that is useful for diagnosis, prognosis, treatment selection and monitoring of tumor.
Contamination of genomic DNA from normal plasma donors can affect accurate measurement of cfDNA(~170bp). Amerigo Scientific offers cfPure DNA-free plasma in which all the DNAs are removed using proprietary technology without affecting other plasma components. The DNA content in cfPure DNA-free plasma is as low as 0 to 10 pg/ml and other plasma components such as total protein, immunoglobulins, and lipids are within the normal range. The DNA-depleted plasma products may be used as a matrix for QC standards to monitor both the specificity and sensitivity of the cfDNA extraction method.