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•  AqueaTether™ Reagents and Kits

Carbohydrates (sugars) are commonly regarded as typical hydrophilic compounds due to the presence of hydroxyl (-OH) groups. Sugar alcohols (SAs), also known as polyols, are chemical compounds containing three or more hydroxyl groups that are formed through the catalytic hydrogenation of carbohydrates. They occur naturally in certain fruits, vegetables, and mushrooms. Polyols are frequently used as sugar-free sweeteners in products such as chewing gum, candies and beverages, or in the manufacture of polymers such as hydrogels.

AqueaTether™ (AqT™) linkers are proprietary chemical architectures assembled from a class of natural and edible sugar alcohol compounds. AqT™ linkers are super-hydrophilic and are designed to label biomolecules with very hydrophobic small molecules, such as fluorescent molecules, biotin, and small molecule drugs.

Sugar alcohols for preparing AqT™ Linkers

3-Carbon	4-Carbon	5-Carbon	6-Carbon
Glycerol	Erythritol Threitol Ribitol Fucitol	Arabitol Xylitol Galactitol	Mannitol Sorbitol

AqT™ Biotin Labeling Products

Biotin is a very hydrophobic molecule that is widely used for protein/antibody labeling (biotinylation) due to its small size, good stability, and interaction with avidin or streptavidin. Antibodies and proteins labeled with biotin tend to precipitate out of solution over time. Therefore, the ratio of labeling is generally 2-4 biotins per antibody/protein for optimized performance.

Modification of biotin using AqT™ linker can increase the hydrophilicity of the biotin, allowing antibodies or proteins to be highly loaded with biotin to enhance/amplify the signal. The hydroxyl groups of the AqT™ linker create a microenvironment that shields neighboring biotins from stacking or interacting with one another. Thus, antibodies/proteins can be labeled with large amounts of AqT™-biotin without changing their properties.

AqT™ ADC Evaluation Kits

AqT™ linkers can be used to conjugate hydrophobic cytotoxic agents with antibodies or proteins to form antibody-drug conjugate (ADC) or protein-drug conjugate (PDC) to improve the overall performance of the ADC or PDC. To facilitate the evaluation of ADCs containing AqT™ linkers, Amerigo Scientific offers kits for evaluating the effect of AqT™ linkers on the in vitro performance of ADCs.

sxLink™ and oxLink™ Protein Crosslinkers

Amerigo Scientific offers proprietary photo-crosslinking reagents for attaching photo-crosslinking groups to biomolecules. These photo-crosslinking reagents contain the carbene-generating phenyldiazirine photoreactive group and the super-hydrophilic AqT™ linker, which gives them significant advantage over conventional photo-crosslinking reagents.