• Amerigo Scientific Instrument
  • Carbohydrates (sugars) are commonly regarded as typical hydrophilic compounds due to the presence of hydroxyl (-OH) groups. Sugar alcohols (SAs), also known as polyols, are chemical compounds containing three or more hydroxyl groups that are formed through the catalytic hydrogenation of carbohydrates. They occur naturally in certain fruits, vegetables, and mushrooms. Polyols are frequently used as sugar-free sweeteners in products such as chewing gum, candies and beverages, or in the manufacture of polymers such as hydrogels.

    AqueaTether™ (AqT™) linkers are proprietary chemical architectures assembled from a class of natural and edible sugar alcohol compounds. AqT™ linkers are super-hydrophilic and are designed to label biomolecules with very hydrophobic small molecules, such as fluorescent molecules, biotin, and small molecule drugs.

    Sugar alcohols for preparing AqT™ LinkersSugar alcohols for preparing AqT™ Linkers

    3-Carbon 4-Carbon 5-Carbon 6-Carbon
    Glycerol Erythritol
    3-Carbon 4-Carbon 5-Carbon 6-Carbon

    AqT™ Biotin Labeling Products

    Biotin is a very hydrophobic molecule that is widely used for protein/antibody labeling (biotinylation) due to its small size, good stability, and interaction with avidin or streptavidin. Antibodies and proteins labeled with biotin tend to precipitate out of solution over time. Therefore, the ratio of labeling is generally 2-4 biotins per antibody/protein for optimized performance.

    Modification of biotin using AqT™ linker can increase the hydrophilicity of the biotin, allowing antibodies or proteins to be highly loaded with biotin to enhance/amplify the signal. The hydroxyl groups of the AqT™ linker create a microenvironment that shields neighboring biotins from stacking or interacting with one another. Thus, antibodies/proteins can be labeled with large amounts of AqT™-biotin without changing their properties.

    Product Name Application Property
    AqT™ Biotin Active Ester Antibody Labeling Kit (T2A15) For generating high-quality antibody with high loading of biotin and minimal or no aggregation Scale of the Reaction: 10 ug-3 mg
    Antibody Labeling Sites: Surface amines
    AqT™ Biotin G/H IgG, Fc Gamma Fragment Specific AqT™ biotinylated antibody for ELISA, western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, and flow cytometry Average Biotin Loading: 4-6 per antibody
    Antibody Labeling Sites: Surface amines

    AqT™ ADC Evaluation Kits

    AqT™ linkers can be used to conjugate hydrophobic cytotoxic agents with antibodies or proteins to form antibody-drug conjugate (ADC) or protein-drug conjugate (PDC) to improve the overall performance of the ADC or PDC. To facilitate the evaluation of ADCs containing AqT™ linkers, Amerigo Scientific offers kits for evaluating the effect of AqT™ linkers on the in vitro performance of ADCs.

    Product Name Application Property
    AqT™ Antibody SN38 Conjugate Evaluation Kit For the evaluation of the benefit of AqT™ linkers for ADCs Scale of the Reaction: 1 or 3 mg
    Antibody Labeling Sites: Surface amines
    Antibody Drug Linkage: Releasable super hydrophilic AqT™ spacer

    sxLink™ and oxLink™ Protein Crosslinkers

    Amerigo Scientific offers proprietary photo-crosslinking reagents for attaching photo-crosslinking groups to biomolecules. These photo-crosslinking reagents contain the carbene-generating phenyldiazirine photoreactive group and the super-hydrophilic AqT™ linker, which gives them significant advantage over conventional photo-crosslinking reagents.

    Product Name Application Property
    Phenyldiazirine oxLink™ (T2A10) Combing the phenyldiazirine group with convenient oxime reversible chemical crosslinking and a super-hydrophilic AqT™ linker Labeling biomolecules containing aldehyde or ketone groups;
    Studying dynamic biomolecule interactions via photo-crosslinking
    Phenyldiazirine sxLink™ (T2A14) Combing the phenyldiazirine group with a cleavable disulfide crosslinking group via a super-hydrophilic AqT™ linker Labeling biomolecules containing free thiol groups;
    Studying dynamic biomolecule interactions via photo-crosslinking

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