With the development of transfection technology and the growing ease of transfection methods, it is very necessary to select a suitable transfection reagent to achieve the optimum transfection efficiency. Transfected cell types and delivered payload should be considered when selecting suitable transfection reagents.

Primary cells are generally more difficult to transfect than cell lines, so transfection reagents are required to facilitate the transfection of payloads into hard-to-transfect cells. The efficiency of our transfection reagents was assessed by using plasmid DNA (pDNA), short interfering RNA (siRNA), messenger RNA (mRNA), and ribonucleoprotein (RNP). These tables below summarize optimal transfection reagents for nucleic acids or proteins in different cell types.

Transfection Reagent Selection

  • Primary Cells

Primary cells are isolated or harvested directly from living tissue or organs. The morphological and physiological characteristics of primary cells are similar to those of the tissue of origin. However, primary cells have a finite lifespan and limited expansion capacity and are usually more difficult to transfect than cell lines. Transfection of primary mammalian cells is an essential tool for scientific and therapeutical applications, such as functional genomics, drug development, and gene-based medicine.

Cell Type All-Fect Trans-Booster Leu-Fect-A Leu-Fect-B Prime-Fect mRNA-Fect CRISP-Fect
Umbilical Cord Blood Derived Mesenchymal Stem Cells (UCB-MSC) pDNA pDNA     pDNA    
mRNA
Bone Marrow Derived Mesenchymal Stem Cells (BM-MSC) pDNA pDNA     pDNA    
mRNA
Vascular smooth muscle Cells (VSMCs)   pDNA     pDNA    
mRNA
Human Umbilical Vein Endothelial Cells (HUVECs)   pDNA          
mRNA
Mononuclear Cells from CML patients (MNC)   pDNA siRNA siRNA      
mRNA
Human Foreskin Fibroblast Cells         pDNA    
Rat Primary Sympathetic Neurons pDNA         mRNA  

  • Cell Lines

Cell lines derived from primary cultures can be propagated repeatedly and sometimes indefinitely. When they are passed on, the cells with the highest growth capacity dominate, resulting in a degree of genotype and phenotypic consistency in the population.

Although cell lines are artificial model systems that do not necessarily reflect the biochemical status of the primary cells, they are primarily used in transfection studies because they are readily available and are generally easier to transfect.

Cell Type All-Fect Trans-Booster Leu-Fect-A Leu-Fect-B Prime-Fect mRNA-Fect CRISP-Fect
Kidney Fibroblast Cells (293-T) pDNA pDNA     pDNA    
mRNA
Breast Cancer Cells (MDA-MB-231) pDNA pDNA   pDNA siRNA mRNA  
siRNA mRNA
co-delivery  
Kidney Epithelial Cells (MDCK)         siRNA    
Breast Cancer/Melanoma Cells (MDA-MB-436)   pDNA     siRNA mRNA RNP
mRNA
Breast Cancer Cells (MDA-MB-468)         siRNA    
Breast Cancer Cells (Sum-149PT)   pDNA     siRNA    
mRNA
Breast Cancer Cells (MCF-7) pDNA pDNA     pDNA mRNA  
mRNA siRNA
Human Lymphoma Cells (U-937) pDNA       pDNA    
Chronic Myeloid Leukaemia Cells (K562) siRNA pDNA siRNA     mRNA  
mRNA
Acute Myeloid Leukemia Cells (KG1 and KG1A) siRNA     siRNA      
Acute Myeloid Leukemia Cells (THP1) siRNA     siRNA   mRNA  
Human Lung Cancer Cells (A549)         siRNA    
Human Colon Cancer (HCT-116) siRNA       siRNA    
Human Myoblasts     ASO        
Jurkat Cells pDNA         mRNA RNP

Benefits of the Reagents

An ideal reagent should have low cytotoxicity and high transfection efficiency for the required cell types. The reagent levels and the cytotoxicity parameters should be considered before selecting an appropriate transfection reagent. Amerigo Scientific offers products that have significant transfection advantages over the main products on the market.

Product Name High Transfection Efficiency Simple Protocol Toxicity
All-Fect Provides 2 to 3-fold higher efficacy in the presences of serum No need to change tissue culture medium during transfection Less toxic compared to commercial transfection reagents, leading to better retention of normal cellular physiology
Prime-Fect Less toxic compared to lipofection reagents, leading to minimal alteration of cell physiology
Leu-Fect-A / Leu-Fect-B Less toxic compared to lipofection reagents, leading to minimal alteration of cell physiology
Trans-Booster Provides 2 to 3-fold higher efficacy in the presences of usual transfection reagent No need to change tissue culture medium during transfection Less toxic compared to lipofection and polymeric reagents, leading to better protein yields from the transfected cells
mRNA-Fect Provides 2 to 7-fold higher efficacy in the presences of serum compared with lipofection reagents No need to change tissue culture medium during transfection  
CRISP-Fect Provides 2 to 7-fold higher efficacy in the presences of serum compared with lipofection reagents No need to change tissue culture medium during transfection Less toxic compared to lipofection reagents, leading to minimal alteration of cell physiology
In Vivo DNA-Fect Provides 2 to 3-fold higher efficacy under serum conditions Can be administered with simple formulations such as saline Less toxic compared to commercial transfecting reagents, leading to better protein yields from the transfected cells
In Vivo RNA-Fect Provides effective transfection under physiological conditions siRNA particles can be prepared in saline suitable for administration  

Application Notes


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