dna stains

Nucleic Acid Stains

The easiest way to see the results of a gel electrophoresis experiment is to stain the gel with a nucleic acid stain. Ethidium bromide (EtBr) is a common stain in agarose and polyacrylamide gels. EtBr binds to DNA molecules by intercalating between adjacent base pairs and emits an intense red-orange fluorescence under UV light. Bands represent the positions of the different size of DNA fragment. However, EtBr is a powerful mutagen with limited sensitivity.

For safety and environmental reasons, non-mutagenic dyes become popular. Most of these dyes can be used as a stain after electrophoresis or as a component in buffer solutions in gel preparation to dissolve agarose or polyacrylamide. Some dyes require UV irradiation to make the bands visible, but others are visualized under other wavelengths of light, for example, blue light, eliminating the danger of possible exposure to UV.

DNA Stains

We offer highly sensitive reagents for staining DNA in electrophoresis gels with higher safety than conventional EtBr staining.

Product Name Target Molecule Size
Novel Juice dsDNA; ssDNA; RNA 1 ml
Novel Green Plus dsDNA 500 μl

Benefits

  • High sensitivity and safety
  • Ready to use
  • Broad compatibility
  • Visualization under blue light or UV
  • No hazardous compounds that require special handling precautions

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