Ion-exchange chromatography (IEX) is a widely used chromatographic method for separating and purifying various types of biological molecules. The separation technology is based on the charge properties and charge quantity of these molecules in a specific buffer system. In the process of capturing target molecules as well as the intermediate purification and polishing of the target biomolecule, the IEX media have the following characteristics:
- High adsorption load and high yield
- Simple operation and low cost of the mobile phase
- Good physical and chemical stability
Amerigo Scientific provides a wide range of Seplife® IEX resins, which are porous spherical media with ion-exchange functional groups and polymer backbone matrix. According to the nature of the functional groups, Seplife® IEX resins are classified as strong acid cation exchange, weak acid cation exchange, strong base anion exchange, and weak base anion exchange.
| Type of IEX Resin |
Functional Group |
Product Model |
| Strong acid cation exchange |
Sulfonate |
SP |
| Weak acid cation exchange |
Carboxymethyl |
CM |
| Strong base anion exchange |
Quaternary ammonium salt |
Q |
| Weak base anion exchange |
Diethylaminoethyl |
DEAE |
In addition, we also offer polymethylacrylate (LXPM) IEX resins and uniform-size polystyrene (LXMS) IEX resins.
Seplife® Agarose IEX Resins
The Seplife® agarose IEX resins can be classified into two main categories, namely the 6AG series and the Large Scale series. Both series contain resins with different particle sizes, functional groups, and capacities.
The Seplife® 6AG agarose resins contain 6% highly cross-linked agarose. Through specific methods, ion exchange functional ligands with different properties are covalently bonded to the agarose matrix, thereby endowing the resins with excellent IEX characteristics.
The Seplife® Large Scale agarose resins are based on matrix beads with high mass transfer performance. Depending on the different particle sizes, the products of this series are further divided into high-rigidity resins (model: Large Scale) and high-rigidity and high-resolution resins (model: Large Scale HP).
| Product |
Ion exchange capacity (mmol/ml) |
Particle size (micron) |
Max. Flow rate (cm/h) |
Max. Pressure resistance (MPa) |
pH Stability |
Application |
| Seplife® 6AG DEAE/90 |
0.11-0.16 (Cl-) |
45-165 |
750 |
0.3 |
4-12 (operational), 3-14 (CIP) |
Fast elution of proteins and mAbs |
| Seplife® 6AG CM/90 |
0.09-0.13 (H+) |
45-165 |
750 |
4-13 (operational), 2-14 (CIP) |
Fast elution of proteins and mAbs |
| Seplife® 6AG Q/200 |
0.18-0.25 (Cl-) |
100-300 |
1800 |
2-12 (operational), 2-14 (CIP) |
Designed for industrial applications, viscous feed (food) and high flow |
| Seplife® 6AG Q/90 |
0.18-0.25 (Cl-) |
45-165 |
750 |
2-12 (operational), 2-14 (CIP) |
Fast elution of proteins and mAbs |
| Seplife® 6AG Q/35 |
0.14-0.20 (Cl-) |
30-50 |
150 |
2-12 (operational), 2-14 (CIP) |
Polishing steps in proteins, mAbs and nucleic acids |
| Seplife® 6AG SP/35 |
0.15-0.20 (H+) |
30-50 |
150 |
4-12 (operational), 3-14 (CIP) |
Polishing steps in proteins and mAbs |
| Seplife® 6AG SP/90 |
0.18-0.25 (H+) |
45-165 |
750 |
4-12 (operational), 3-14 (CIP) |
Fast elution of proteins and mAbs |
| Seplife® Q Large Scale/90 |
0.16-0.22 (Cl-) |
45-165 |
1000 |
2-12 (operational), 2-14 (CIP) |
High flow and high DBC for proteins and mAbs purification |
| Seplife® S Large Scale/90 |
0.11-0.14 (H+) |
45-165 |
500 |
4-12 (operational), 3-14 (CIP) |
High flow and high DBC for proteins and mAbs purification |
| Seplife® SP Large Scale HP/40 |
0.13-0.16 (H+) |
30-50 |
400 |
4-12 (operational), 3-14 (CIP) |
Polishing steps in proteins and mAbs |
Characteristics of the IEX Agarose Resins
- The hydrophilic matrix ensures extremely low non-specific adsorption levels and high recovery rates.
- The particle size distribution of the resins covers a range from an average diameter of 30-300 μm, making this product series suitable for a wide range of applications.
- The IEX agarose resins have good stability against alkaline (up to 1M sodium hydroxide) for CIP.
- Regulatory Support File (RSF) can be provided.
Seplife® Dextran IEX Resins
The Seplife® dextran IEX resins are ion exchange chromatographic media based on highly cross-linked dextran bonded to weak base anion (DEAE) or weak acid cation (CM) functional groups, with a particle size range of 40-120 μm. The product name includes the abbreviation of the functional group, and the numbers in the name represent the composition of the dextran.
- Seplife® Dextran IEX resins are designed for the chromatographic purification of biomolecules.
- Resins are provided in dry form.
- Regulatory Support File (RSF) can be provided.
| Product |
Ion exchange capacity (mmol/g) |
Particle size (micron, dry) |
Max. Flow rate (cm/h) |
pH Stability |
Application |
| Seplife® DX DEAE 25/80 |
3.0 -4.0 (Cl-) |
40-120 |
480 |
2-9 (operational), 2-13 (CIP) |
For the separation of smaller molecules and proteins (up to 30KDa) in column mode |
| Seplife® DX DEAE 50/80 |
3.0 -4.0 (Cl-) |
60 |
2-9 (operational), 2-12 (CIP) |
For the separation of large proteins (up to 100KDa) in batch mode. |
| Seplife® DX CM 25/80 |
4.0-5.0 (H+) |
600 |
6-10 (operational), 2-13 (CIP) |
For the separation of smaller molecules and proteins (up to 30KDa) in column mode. |
Seplife® Polymeric IEX Resins
Seplife® synthetic polymer IEX resins with the robust polystyrene or polymethacrylate backbone are suitable for the efficient treatment of various molecules ranging from small molecules to large molecules. These resins are ideal tools for key separation of biological molecules.
Seplife® LXPM Resins: They are a type of polymethyl methacrylate IEX resins, which are made using a unique polymer synthesis technology. Their surfaces are modified with hydrophilic long-chain macromolecules and coupled with different ion-exchange groups. Due to their good hydrophilicity, chemical and physical stability, as well as a robust structure, Seplife® LXPM resins have good biocompatibility and service life, enabling improved purification efficiency.
Seplife® LXMS Resins: They are a type of polystyrene IEX resins with uniform particle size. The highly cross-link property enables the resins to withstand high operating pressure (3MPa).
Seplife® polymeric IEX resins have the characteristics of high consistency and reliability, maintaining stability throughout the pH range (0 to 14) and performing well under most solvent conditions. Both Seplife® LXMS resin and Seplife® LXPM resin are suitable for the capture of molecules such as antibodies, proteins, peptides, nucleic acids, antibiotics and natural products, as well as for intermediate purification and fine purification applications.
| Product |
Ion exchange capacity (mmol/ml) |
Particle size (micron) |
Max. Flow rate (cm/h) |
pH Stability |
Application |
| Seplife® LXPM SP706M |
0.05-0.08 |
50-100 |
500-600 |
3-12 (operational), 3-14 (CIP) |
For use in capture and polishing purification of medium size biomolecules |
| Seplife® LXPM SP5504M |
0.05-0.11 |
50-100 |
500-600 |
For use in capture and polishing purification of medium size biomolecules |
| Seplife® LXPM SP5504C |
0.05-0.11 |
100-300 |
600-700 |
For use in the capture purification of medium size biomolecules |
| Seplife® LXMS 30S |
0.07-0.10 |
500 |
900 |
2-12 (operation), 1-14 (CIP) |
For use in polishing steps with or without organic solvents and at high flow rates |
| Seplife® LXMS 30QN |
0.07-0.10 |
30±3 |
100-900 |
For polishing of antibodies, nanobodies, proteins, peptides, and nucleic acids, as well as the purification of small molecules |
| Seplife® LXMS 15S |
0.07-0.10 |
500 |
600 |
For use in polishing steps with or without organic solvents and at high flow rates |
| Seplife® LXMS 15QN |
0.15-0.20 |
15±2 |
100-600 |
For polishing of antibodies, nanobodies, proteins, peptides, and nucleic acids, as well as the purification of small molecules |
| Seplife® LX-AG1×8 (Cl form) |
≥1.5 |
100-300 |
|
1-14 |
For peptide salt conversion or for analytical applications |
| Seplife® LX-AG1×8 (OH form) |
| Seplife® LX-AG1×8 (Acetate form) |
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