MicroStacker™ Detection Kits are immunohistochemistry (IHC) detection tools based on micro-polymer stacking technology. They are developed by integrating core technologies including micro-polymer scaffolds, HRP stacking, Fab' labeling, and advanced conjugation chemistry.
Amerigo Scientific offers MicroStacker™ Detection kits that are biotin-free secondary antibody conjugation systems designed for the detection of primary antibodies on tissues during the IHC process (regular IHC, multiplex IHC, and intraoperative frozen-section IHC). By eliminating the need for streptavidin or biotin, it effectively avoids non-specific staining.
MicroStacker™ Technology
MicroStacker™ technology enables precise control over the layered stacking of F(ab') fragments of IgG secondary antibody and peroxidase enzymes onto a micro-polymer scaffold.
- The compact micro-polymer structure easily penetrates all cellular compartments, ensuring consistent staining of nuclear, cytoplasmic, and membrane antigens. This provides superior sensitivity and specificity compared to traditional HRP polymers that utilize bulky dextran backbones.
- By utilizing F(ab') fragments instead of whole IgG, the technology eliminates background interference caused by the non-specific binding of IgG to endogenous Fc receptors.
- Novel chemical conjugation techniques ensure higher reagent stability.
Manual MicroStacker™ Detection Kits
| Product |
MicroStacker™ Polymer Detection Kit, Universal |
MicroStacker™ Polymer Detection Kit, Enhanced |
| Advantages |
High sensitivity, stable reagents. |
Compared to MicroStacker™ Universal, the Enhanced detection achieves higher sensitivity because the additional amplification step further amplifies the primary antibody signal. |
| Applications |
Suitable for manual IHC detection of mouse and rabbit antibodies in paraffin-embedded tissues. |
Suitable for manual IHC detection of mouse and rabbit antibodies in paraffin-embedded tissues.
Suitable for low-expression antigens and low-affinity antibodies. |
| Staining Procedure |
Primary Ab Incubation: RT, 60 min;
Secondary Ab Incubation: RT, 30 min;
DAB Incubation: 5 min |
Primary Ab Incubation: RT, 30 min;
Signal Amplification: RT, 30 min;
Secondary Ab Incubation: RT, 30 min;
DAB Incubation: 5 min |
| Application Example |
HER2 (Rabbit mAb) on Breast Tissue |
HER2 (Rabbit mAb) on Breast Tissue |
On-board MicroStacker™ Detection Kits
| Product |
MicroStacker™ Flex HRP-Polymer Detection Kit |
MicroStacker™ Plus HRP-Polymer Detection Kit |
| Advantages |
Flexible selection of post-primary amplifiers to further enhance staining results |
The increased polymerization of enzymes and antibodies, achieving higher sensitivity and faster staining (saving approx. 35 min) |
| Applications |
Automated IHC staining for CNT300/CNT330 equipment |
Automated IHC staining for CNT360 equipment |
| Staining Procedure |
Primary Ab Incubation: RT, 30min;
Signal Amplification: RT, 20min;
Secondary Ab Incubation: RT, 20min;
DAB: 5min. |
Primary Ab Incubation: RT, 20min;
Signal Amplification: RT, 8min;
Secondary Ab Incubation: RT, 8min;
DAB: 5min. |
| Application Example |
P40 on Lung Squamous Cell Carcinoma Tissue |
MUC2 on Intestinal Tissue |
Other MicroStacker™ Detection Kits
MicroStacker™ Rabbit-on-Rodent HRP-polymer detection kit is mainly used for the staining of rabbit monoclonal/polyclonal antibodies on mouse/rat tissues. MicroStacker™ goat anti-rabbit HRP-polymer detection kit and MicroStacker™ goat anti-mouse HRP-polymer detection kit are respectively used for the detection of rabbit monoclonal/multiclonal antibodies and mouse monoclonal/polyclonal antibodies in human tissues. MicroStacker™ Mouse-on-Mouse polymer detection kit is used for the superior detection of mouse primary antibodies on mouse tissue, providing lower background and clearer staining compared to conventional methods.
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