Biotin-Free IHC Detection Kits with Micro-Polymer Stacking Technology

MicroStacker™ Detection Kits are immunohistochemistry (IHC) detection tools based on micro-polymer stacking technology. They are developed by integrating core technologies including micro-polymer scaffolds, HRP stacking, Fab' labeling, and advanced conjugation chemistry.

Amerigo Scientific offers MicroStacker™ Detection kits that are biotin-free secondary antibody conjugation systems designed for the detection of primary antibodies on tissues during the IHC process (regular IHC, multiplex IHC, and intraoperative frozen-section IHC). By eliminating the need for streptavidin or biotin, it effectively avoids non-specific staining.

MicroStacker™ Technology

MicroStacker™ technology enables precise control over the layered stacking of F(ab') fragments of IgG secondary antibody and peroxidase enzymes onto a micro-polymer scaffold.

• The compact micro-polymer structure easily penetrates all cellular compartments, ensuring consistent staining of nuclear, cytoplasmic, and membrane antigens. This provides superior sensitivity and specificity compared to traditional HRP polymers that utilize bulky dextran backbones.
• By utilizing F(ab') fragments instead of whole IgG, the technology eliminates background interference caused by the non-specific binding of IgG to endogenous Fc receptors.
• Novel chemical conjugation techniques ensure higher reagent stability.

Manual MicroStacker™ Detection Kits

Product	MicroStacker™ Polymer Detection Kit, Universal	MicroStacker™ Polymer Detection Kit, Enhanced
Advantages	High sensitivity, stable reagents.	Compared to MicroStacker™ Universal, the Enhanced detection achieves higher sensitivity because the additional amplification step further amplifies the primary antibody signal.
Applications	Suitable for manual IHC detection of mouse and rabbit antibodies in paraffin-embedded tissues.	Suitable for manual IHC detection of mouse and rabbit antibodies in paraffin-embedded tissues. Suitable for low-expression antigens and low-affinity antibodies.
Staining Procedure	Primary Ab Incubation: RT, 60 min; Secondary Ab Incubation: RT, 30 min; DAB Incubation: 5 min	Primary Ab Incubation: RT, 30 min; Signal Amplification: RT, 30 min; Secondary Ab Incubation: RT, 30 min; DAB Incubation: 5 min
Application Example	HER2 (Rabbit mAb) on Breast Tissue	HER2 (Rabbit mAb) on Breast Tissue

On-board MicroStacker™ Detection Kits

Product	MicroStacker™ Flex HRP-Polymer Detection Kit	MicroStacker™ Plus HRP-Polymer Detection Kit
Advantages	Flexible selection of post-primary amplifiers to further enhance staining results	The increased polymerization of enzymes and antibodies, achieving higher sensitivity and faster staining (saving approx. 35 min)
Applications	Automated IHC staining for CNT300/CNT330 equipment	Automated IHC staining for CNT360 equipment
Staining Procedure	Primary Ab Incubation: RT, 30min; Signal Amplification: RT, 20min; Secondary Ab Incubation: RT, 20min; DAB: 5min.	Primary Ab Incubation: RT, 20min; Signal Amplification: RT, 8min; Secondary Ab Incubation: RT, 8min; DAB: 5min.
Application Example	P40 on Lung Squamous Cell Carcinoma Tissue	MUC2 on Intestinal Tissue

Other MicroStacker™ Detection Kits

MicroStacker™ Rabbit-on-Rodent HRP-polymer detection kit is mainly used for the staining of rabbit monoclonal/polyclonal antibodies on mouse/rat tissues. MicroStacker™ goat anti-rabbit HRP-polymer detection kit and MicroStacker™ goat anti-mouse HRP-polymer detection kit are respectively used for the detection of rabbit monoclonal/multiclonal antibodies and mouse monoclonal/polyclonal antibodies in human tissues. MicroStacker™ Mouse-on-Mouse polymer detection kit is used for the superior detection of mouse primary antibodies on mouse tissue, providing lower background and clearer staining compared to conventional methods.