Seplife® DX DEAE 25/80 Dextran-Based Chromatography Resin

Dextran-Based Chromatography Resins

Appearance: White spherical beads
Type: Weak base anion - Diethylaminoethyl
Matrix: Crossed linked dextran
Ion exchange capacity (mmol/g): 3.0 -4.0(Cl-)
Particle size (dry, μm): 40-120
Swelling property (ml/g): 5.0-7.0
pH stability: 2-9 (operational), 2-13 (CIP)
Chemical Stability: Stable in all common aqueous buffers; 1M sodium hydroxide; 8M urea; 6M guanidine hydrochloride; 70% ethanol.
Flow rate* (cm/h): max. 480
Shipped as: Dry

*Testing conditions: Chromatography column 16mm×200mm; column bed height 15cm; temperature 25°C; mobile phase 0.1M NaCl.

Seplife®DX DEAE 25/80 is provided as a dry powder and needs to be swollen when used. The swelling ratio depends on the buffer solution used. The swelling ratio can vary significantly in different solution compositions. Do not use magnetic stirring during swelling, to avoid breaking the particles.
Product pretreatment Weigh the required amount of Seplife®DX DEAE 25/80, add 50~100 times distilled water or equilibration buffer, and let it swell. Swelling typically takes 1~2 days at room temperature, or 2 hours in boiling water.

Column packing
Column packing should be done according to standard operating procedures. It is important to ensure that each material is at its working temperature, and the chromatography media may need to be degassed before column packing.
Note
If the column is packed at the maximum linear flow rate, the flow rate during the subsequent chromatographic separation should not exceed 75% of the column packing flow rate.

Equilibration
Equilibrate the column with an appropriate 2-5 column volume buffer. Ensure the conductivity and pH of the effluent are the same as the buffer.

Sample feeding
Determine the loading amount according to the target product concentration and the loading capacity of the media. Samples with particulates and precipitate should be filtered or centrifuged before the chromatography purification.
Cleaning
After loading the sample, equilibrate the column with loading buffer to wash away unbound proteins and impurities until the conductivity and pH of the effluent are exactly the same as those of the loading buffer.

Elution
Use continuous or gradient elution with increasing salt concentration in the buffer or decreasing pH.

Regeneration
First wash off the impurity proteins on the column with 1~2M NaCl. Then wash off the salt from the column with distilled water, to stabilized the conductivity and pH of the effluent. Then pass the loading buffer through the column with until the conductivity and pH of the effluent are stable.

Storage
Seplife®DX DEAE 25/80 dry powder should be stored in a dry, ventilated and clean place at 4~30°C; the hydrated media should be stored in 20% ethanol solution or 0.1M NaOH at 4~8°C.