AQuora® Dyes
AQuora® dyes feature a hydrophilic PEG coating, which enables high water solubility, high labeling degree, and prevents non-specific interactions and self-quenching of the resulting conjugate. Therefore, AQuora® dyes are highly suitable for complex applications requiring maximum signal strength and minimal background interference. The dyes are available as NHS ester and maleimide forms, and custom reactive groups can be provided upon request.
- Conjugated with PEG spacers, AQuora® dyes have the characteristics of increased solubility, decreased non-specific binding, enabling an extremely high labeling degree (DOL) and achieving very low background.
- AQuora® dyes yield enhanced signal and signal-to-noise ratios in fluorescence-based applications, including fluorescent western blotting, fluorescence-based microscopy, flow cytometry, and cell-based assays.
- AQuora® NHS Ester dyes are amine-reactive dyes that can be conjugated to many proteins or peptides. The NHS ester reacts with primary amines to form a stable, covalent amide bond.
- AQuora® Maleimide dyes can be conjugated to many proteins or peptides through cysteine thiol groups. The maleimide group reacts with thiols to form thioether bonds.
Both NHS Ester reagents and Maleimide reagents are moisture-sensitive, and the dyes are light- and oxygen-sensitive. Therefore, the AQuora® dyes must be protected from exposure to light and should be blanketed with dry, inert gas, such as nitrogen or argon, whenever they are open or in use.
AQuora® Dye Products
| Fluorophore | Core Dye Excitation | Core Dye Emission | Extinction Coefficient | NHS Ester Dyes | Maleimide Dyes |
| AQuora 350 | 347 | 452 | 19,000 | AQuora® 350-NHS Ester | AQuora® 350-Maleimide |
| AQuora 405 | 399 | 422 | 35,000 | AQuora® 405-NHS Ester | AQuora® 405-Maleimide |
| AQuora PB | 404 | 458 | 19,000 | AQuora® PB NHS Ester (Pacific Blue) | AQuora® PB-Maleimide (Pacific Blue) |
| AQuora 488 | 493 | 522 | 73,000 | AQuora® 488-NHS Ester Solution | AQuora® 488-Maleimide |
| AQuora Fluorescein | 495 | 524 | 76,000 | AQuora® Fluorescein-NHS Ester | AQuora® Fluorescein-Maleimide |
| AQuora TAMRA | 544 | 582 | 92,000 | AQuora® TAMRA NHS Ester | AQuora® TAMRA-Maleimide |
| AQuora 550 | 554 | 570 | 150,000 | AQuora® 550-NHS Ester | AQuora® 550-Maleimide |
| AQuora TX595 | 592 | 611 | 80,000 | AQuora® TX595-NHS Ester | AQuora® TX595-Maleimide |
| AQuora 650 | 654 | 674 | 250,000 | AQuora® 650-NHS Ester | AQuora® 650-Maleimide |
| AQuora 750 | 755 | 780 | 240,000 | AQuora® 750-NHS Ester | AQuora® 750-Maleimide |
| AQuora 800 | 792 | 809 | 270,000 | AQuora® 800-NHS Ester | AQuora® 800-Maleimide |
Product Properties
Figure1. Excitation Wavelength of AQuora® Dyes
| Excitation/Emission Range: | UV to Near IR (350-800 nm) |
| Photostability: | High |
| Brightness: | High |
| Options for Near Infrared Imaging: | Yes |
| pH Stability: | pH 4-9 |
| Hydrophilicity: | Very High (dPEG technology) |
| Degree of Labeling (DOL) Flexibility: | Very High |
| Fluorescent only when conjugated: | No |
Product Applications
- In Vivo Applications (esp. NIR Dyes)
- Live Cell Imaging
- Immunofluorescence
- Flow Cytometry
- ELISA
- Subcellular Localization
- Multiplexing
- FRET (Limited)
- Oligonucleotide Labeling
- Peptide/Protein Labeling
Superior Quality and Consistency
Our AQuora® dyes exhibit outstanding performance backed by detailed quality documentation and batch records, high product purity, reliable batch-to-batch consistency, and technical support. The dyes are produced with a purity exceeding 90%, making them a reliable choice when considering the performance repeatability. Each batch of each dye comes with a certificate of analysis to ensure quality consistency between batches. Moreover, highly qualified, bench-trained technical support personnel will assist you in solving problems and optimizing work processes.
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