Seplife® DX DEAE 50/80 Dextran-Based Chromatography Resin

Dextran-Based Chromatography Resins

Appearance: White spherical beads
Type: Weak base anion - Diethylaminoethyl
Matrix: Crossed linked dextran
Ion exchange capacity (mmol/g): 3.0 -4.0(Cl-)
Particle size (dry, μm): 40-120
Swelling property (ml/g): 75.0-95.0
pH stability: 2-9 (operational), 2-12 (CIP)
Chemical Stability: Stable in all common aqueous buffers; 1M sodium hydroxide; 8M urea; 6M guanidine hydrochloride; 70% ethanol. Avoid exposure to strong oxidizing agents and dextranase.
Flow rate* (cm/h): max. 60
Shipped as: dry

*Testing conditions: Chromatography column 16mm×200mm; column bed height 5cm; temperature 25°C; mobile phase 0.1M NaCl

Seplife®DX DEAE 50/80 is provided as a dry powder and needs to be wetted and swollen before use. The swelling ratio depends on the buffer solution used and can vary significantly.
Due to the large variation in volume depending on the buffer composition, Seplife®DX DEAE 50/80 may be more suitable for applications in batch mode.
Do not use magnetic stirring during processing, to avoid breaking the particles.
Product pretreatment Weigh the required amount of Seplife®DX DEAE 50/80, add 50~100 times distilled water or equilibration buffer solution and let it swell. Swelling typically takes 1~2 days at room temperature, or 2 hours in boiling water.

Column packing
Column packing should be done according to standard operating procedures. It is important to ensure that each material is at its working temperature, and the chromatography media may need to be degassed before column packing.
Note: If the column is packed at the maximum linear flow rate, the flow rate during the subsequent chromatographic separation should not exceed 75% of the column packing flow rate.

Equilibration
Equilibrate the column with an appropriate 2-5 column volume buffer. Ensure the conductivity and pH of the effluent are the same as the buffer.

Sample feeding
Determine the loading amount according to the target product concentration and the loading capacity of the media. Samples with particulates and precipitate should be filtered or centrifuged before the chromatography purification.
Cleaning
After loading the sample, equilibrate the column with loading buffer to wash away unbound molecules until the conductivity and pH of the effluent are the same as for the loading buffer.

Elution
Use continuous or gradient elution with increasing salt concentration in the buffer or decreasing pH.

Regeneration
First wash off the impurity proteins on the column with 1~2M NaCl. Then wash off the salt from the column with distilled water.

Storage
Seplife®DX DEAE 50/80 dry powder should be stored in a dry, ventilated and clean place at 4~30°C; the hydrated media should be stored in 20% ethanol solution or 0.1M NaOH at 4~8°C to control microbial growth.