3D TableTrix™ and 3D RecomTrix™ Microcarriers - Pharmaceutical Grade

Amerigo Scientific offers 3D TableTrix™ and 3D RecomTrix™ microcarriers that are made from safe and common biological materials, and their design aims to optimize cell growth while addressing key challenges in the cell manufacturing process. Unlike traditional static or suspension methods, our microcarriers provide a dynamic solution for scaling adherent cell cultures.

The 3D TableTrix™ and 3D RecomTrix™ microcarriers are specially designed to gently dissolve under controlled conditions, achieving nearly 100% cell recovery and minimal residual. Using the microcarriers ensures high cell survival rates, simplifies the harvesting process, and reduces the risk of contamination, which are crucial for obtaining high-quality cell therapeutic products. In addition, the porous structure of our microcarriers provides a large surface area, enabling greater cell expansion within the same cell culture system volume, thereby increasing production efficiency and reducing costs.

Why Choose 3D TableTrix™ and 3D RecomTrix™ Microcarriers?

The use of 3D TableTrix™ and 3D RecomTrix™ microcarriers can accelerate the development of gene cell therapies. DMFs have been registered with the FDA.

• Type IV DMF Number: 035481 (Pharmaceutical Excipient)
• Type II DMF Number: 037798 (Starting Materials)
• Type II CBER MF Number: 29721 (Starting Materials)

3D RecomTrix® Recombinant Collagen Microcarriers

3D RecomTrix® microcarriers are biomimetic and elastic porous microcarriers that are made from recombinant type I collagen protein and suitable for large-scale expansion and culture of adherent cells. The 3D RecomTrix® microcarriers feature a large specific surface area and high uniformity, which can simulate the growth environment of cells in the body and significantly improve the quality and efficiency of cell culture.

3D RecomTrix® microcarriers can be used for large-scale and standardized culture and harvest of most adherent cells (especially mesenchymal stem cells) and their products. The products are pre-sterilized and ready-to-use, convenient to use, and can achieve gentle cell harvest with specific degradation technology.

3D TableTrix® Microcarriers

The 3D TableTrix® microcarrier consists of tens of thousands of elastic 3D porous microcarriers with porosity >90%, particle size range of 50-500 μm, and a large specific surface area enabling high-density cell production. Featuring uniformity ≤100 μm and proprietary degradation technology, 3D TableTrix® microcarrier allows gentle harvesting of cells. 3D TableTrix® microcarrier includes W, V, and G series - specifically recommended for cell therapy, vaccine development, and gene therapy applications respectively, based on their distinct properties.

Product Features of 3D TableTrix™ and 3D RecomTrix™ Microcarriers

• >90% porosity and elasticity, replicating the native cell microenvironment.
• Shear-safe design, shielding cells from stress (<10s^-1)
• Sterile, ready-to-use tablets ideal for small-scale R&D, or bulk systems available for large-scale production
• Tablets (powder) instantly dispersing into thousands of microcarriers upon hydration, streamlining process development
• Fully dissolvable microcarriers that are gently dissolved at 37°C without harsh chemicals, enabling efficient, high-yield cell recovery

Figure 1. Unique tablet design that disperses instantly in water

Figure 2. 3D TableTrix® and 3D RecomTrix® microcarrier developed based on proprietary degradation technology, enabling efficient cell harvesting

Application Case 1: Vero Cell Culture

Vero cells derived from African green monkey kidney cells are used primarily for viral vaccine production and research. Using 3D TableTrix™ microcarrier, continuous multi-stage scale-up subculture of Vero cells in a cell culture system could be enabled with a peak cell density of 1×10⁷/mL. Titer and efficacy of viruses produced from cells cultured on 3D TableTrix™ microcarrier were significantly higher than those from 2D culture or traditional spherical microcarriers.

Application Case 2: Mesenchymal Stromal Cell Culture

Mesenchymal stromal cells (MSCs) are spindle-shaped adherent cells isolated from bone marrow, adipose, and other tissues, and possess the capacity for multipotent differentiation in vitro. MSCs play a crucial role in regenerative medicine due to their potential to repair tissues and immunosuppressive properties.

Multiple cases showed that MSCs could be expanded up to 10-16 folds on 3D TableTrix™ Microcarriers. Expansion remained stable at 12-14 folds across 6 continuous subcultures. Using 3D TableTrix™ microcarriers, 3-phase scale-up production of 10 billion cells per batch was achieved in the cell culture system.