Human platelet lysate (hPL) is a source of various growth factors and other bioactive proteins, which are involved in the processes of cell growth, proliferation, and differentiation. Therefore, hPL has been used in various clinical research, tissue engineering, and other fields. Human methacryloyl platelet lysate (hPLMA) is a bioactive derivative precursor of hPL, and it can be cured under light exposure to form a hydrogel with adjustable mechanical properties. The hydrogel can be used to culture different human-derived cells to support important biological processes such as cell growth, germination, and migration.
hPLMA Sponges
Amerigo Scientific provides hPLMA Sponge, which is made from human methacrylated platelet lysate. The hPLMA Sponge is a ready-to-use porous scaffold specifically designed for 3D cell culture applications. It is manufactured from platelet units obtained from screened healthy human donors designated for therapeutic transfusion.
The hPLMA Sponge scaffold provides a xeno-free, biocompatible microenvironment that supports cell attachment, proliferation, and differentiation even without traditional animal derived supplementation. This porous scaffold has high reproducibility and a large surface area and is suitable for standard 96-well plates.
Figure 1. The preparation of hPLMA Sponge
Identified Growth Factors
hPLMA Sponge is composed of various growth factors and cytokines. The identified growth factors include platelet-derived growth factor (PDGF), vascular endothelial-derived growth factor (VEGF), erythropoietin (EPO), epidermal growth factor (EGF), and hepatocyte growth factor (HGF). These bioactive proteins are known to be key components involved in cell adhesion and proliferation processes, and are also major regulators of angiogenesis.
Applications
The hPLMA Sponge provides a xeno-free platform with biocompatibility, enabling cell attachment, proliferation, and differentiation even without traditional animal-derived supplements. This makes hPLMA Sponge particularly suitable for various applications including drug screening, disease modeling, and the development of tissue engineering structures. In the fields of tissue engineering and regenerative medicine, the microenvironment of 3D cell culture can simulate the natural in vivo environment, enabling the accurate study of cell behavior, interactions and responses, which is something that the traditional 2D culture microenvironment cannot achieve.
Porous Structure
Figure 2. Internal structure of hPLMA Sponge scaffolds. (Scale bar: 300μm)
Our hPLMA Sponge possesses porous internal structure and biological activity. Its pore size is around 25±5 µm. The maximum swelling of the sponges is achieved after approximately 5 min (in PBS) and they present a Young modulus' around 13 KDa. The porous structure of the hPLMA Sponge facilitates the effective infiltration of cells, the diffusion of nutrients, and the excretion of waste, which is crucial for maintaining the vitality and functionality of cells in 3D culture.
hPLMA Sponge for hASCs 3D Culture
Human adipose-derived stem cells (hASCs) can adhere and proliferate continuously on hPLMA Sponge scaffold for up to 14 days, even without supplementing animal-derived serum. Figure A shows Live/Dead and DAPI/Phalloidin staining (scale: 100 micrometers) of hASCs on the hPLMA Sponge after 14 days of culture in the presence and absence of fetal bovine serum (FBS), respectively.
In Figures B and C, the DNA results and MTS results of hASCs after 3 days, 7 days and 14 days of cultivation under the conditions with and without FBS were compared respectively. Statistical analysis through two-way ANOVA combined with Tukey's multiple comparisons test showed significant differences between the analyzed groups: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
Figure 3. hASCs cell culture in hPLMA Sponges
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