Viral plasmids are engineered DNA vectors designed and constructed based on viral genome structures for efficient gene delivery, expression, or genome modification in cells, by constructing viral vectors or producing viral particles. The plasmids typically retain key elements necessary for viral replication, packaging, or transcription while deleting pathogenic or replication-related sequences, ensuring high transduction efficiency and safety. Leveraging the inherent biological characteristics of viruses, viral plasmids have become indispensable tools in molecular biology, gene therapy research, and cell engineering experiments. They can be used in various applications, such as gene function research, CRISPR editing system construction, stable cell line construction, vaccine development, and in vitro and in vivo gene delivery.
The main characteristics of viral plasmids include high-efficiency expression, high customizability, and controllable safety. They typically contain strong promoters, enhancers, and multiple cloning sites, allowing for flexible insertion of target genes or regulatory sequences. Furthermore, their structure is optimized to remove viral replication capabilities, preventing the plasmid itself from forming infectious viruses in the host. To meet different experimental needs, viral plasmids can also carry fluorescent markers, resistance genes, or selection tags, further enhancing experimental visualization and operability.
Different viral systems correspond to different plasmid structures with their own regulatory characteristics, allowing researchers to choose the most suitable vector platform according to their needs.
- Adeno-associated virus (AAV) plasmids: One of the most widely used types in gene therapy research, with low pathogenicity and broad tissue adaptability.
- Lentiviral plasmids: Capable of integrating into the host genome to achieve long-term and stable gene expression, commonly used for generating stable cell lines.
- Adenoviral plasmids: Characterized by very high expression efficiency and high viral yield, suitable for vaccine development and studies requiring short-term high-level expression.
- Retroviral plasmids: Used for genetic modification of dividing cells, especially suitable for specific experimental models that rely on cell proliferation.
Amerigo Scientific offers a diverse range of viral plasmids designed for the development of biopharmaceuticals, delivery technologies, and research in molecular biology, etc.
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