High-quality nucleic acid preparation is essential for obtaining accurate and reproducible results in modern molecular biology workflows. From sample collection to downstream analysis, improper handling or degradation of nucleic acids can significantly impact data reliability, particularly in sensitive applications such as gene expression analysis and next-generation sequencing (NGS).
Nucleic acid preparation solutions are designed to address key challenges throughout the sample preparation process, including nucleic acid stabilization, efficient extraction, and removal of unwanted contaminants. By integrating optimized chemistries and streamlined workflows, these solutions help preserve nucleic acid integrity while improving overall yield and purity.
FavorPrep™ RNA Isolation Solution
Amerigo Scientific offers FavorPrep™ Tri-RNA Reagent for rapid, one-step isolation of high-quality total RNA. Formulated with phenol and guanidine isothiocyanate, it efficiently lyses samples while preserving the RNA integrity even in the smallest molecular size.
Features
- Single step for the isolation of total RNA from tissues, cells, bacteria, plants, yeasts and biological fluids
- The entire procedure for total RNA isolation is less than 1 hour.
- Available for the isolation of RNA, DNA and protein.
- The purified RNA can be applied in: RT-PCR, Northern hybridization, RNase protection, Poly-A+RNA selection, Differential display, and Micro-array assay.
Protocol Overview
FavorPrep™ DNA/RNA Stabilization Solution
FavorPrep™ RNA Stabilization Solution and FavorPrep™ NApreserve Reagent are designed to preserve nucleic acids immediately after sample collection. They effectively prevent RNA and DNA degradation, enabling reliable downstream molecular analyses. These solutions are compatible with a wide range of biological materials, including cells, tissues, and fluids, and simplify sample handling and storage without the need for immediate processing.
Features
- Broad Sample Compatibility: Effective for cells, tissues, stool, soil, and other biological materials.
- Rapid and Convenient: Simplifies sample handling and preserves nucleic acids immediately after collection.
- High-Quality Preservation: Maintains nucleic acid integrity for downstream applications such as PCR, hybridization, and sequencing.
Protocol Overview
Rapid Workflow for RNA Stabilization and High-Quality RNA Recovery
Convenient Workflow for Ambient Nucleic Acid Preservation and Transport
FavorPrep™ Short-DNA Removal Solution
Amerigo Scientific offers FavorPrep™ Short-DNA Depleter for the efficient removal of DNA fragments smaller than 10 kbp while enriching high–molecular–weight (HMW) DNA. This product is ideal for preparing intact long DNA for shearing and library construction in long-read sequencing platforms such as PacBio® SMRT and Oxford Nanopore Technologies®.
Features
- Safe and convenient: Composed of non-toxic reagents and allowing simple, column-free processing for isolating large DNA fragments with high purity.
- Reliable Size Selection: Optimized formulation ensures consistent depletion and easy DNA recovery via centrifugation or standard precipitation.
Protocol Overview
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