1-Step RT-qPCR Solutions for Advanced Life Science Research

Amerigo Scientific is dedicated to accelerating discovery by equipping research institutions with state-of-the-art molecular biology reagents. We offer a comprehensive portfolio of high-performance 1-Step RT-qPCR Kits, engineered to streamline RNA analysis workflows while delivering uncompromising sensitivity and specificity.

Designed strictly for research applications, these kits consolidate reverse transcription and quantitative PCR into a single, closed-tube reaction. This minimizes the risk of cross-contamination, reduces hands-on pipetting time, and ensures highly reproducible data for gene expression analysis, viral RNA research, and copy number variant studies.

These 1-Step RT-qPCR kits are powered by a synergistic blend of proprietary enzymes and optimized buffers:

• Thermostable Reverse Transcriptase & RNase Protection: Features an engineered MMuLV reverse transcriptase that remains highly active and thermostable up to 55°C. This allows for the efficient processing of complex, secondary structure-rich, or long RNA templates. An advanced RNase Inhibitor is integrated to safeguard target RNA integrity from enzymatic degradation.
• Small-Molecule Hot Start Technology: Amplification is driven by a proprietary Hot Start Taq DNA Polymerase. Enzyme activity is strictly inhibited at room temperature via a small molecular inhibitor and is only activated upon initial heating. This completely prevents non-specific amplification and primer-dimer formation, ensuring zero background noise.
• Broad Instrument Compatibility: To accommodate the diverse thermal cycler platforms used across research core facilities, our master mixes are available in No ROX, Low ROX, and High ROX formulations. Nuclease-free PCR Water is included in all kits to guarantee optimal reaction conditions.

Dye-Based/Probe-Based 1-Step RT-qPCR Kits

1-Step RT-PCR Kits

For researchers requiring downstream cloning or gel analysis, our standard 1-Step RT-PCR Kit provides highly specific endpoint amplification. The proprietary Hot Start Taq produces A-tailed PCR products, seamlessly ready for subsequent TA cloning vector ligation.

1-Step RT-qPCR Green Kits

1-Step RT-qPCR Green Kits are ideal for robust, standard dye-based expression analysis. These kits deliver rapid extension rates and early Ct values under both standard and fast cycling conditions. They provide excellent amplification efficiency, even when targeting challenging AT/GC-rich templates.

2X 1-Step RT-qPCR Probe Kits

2X 1-Step RT-qPCR Probe Kits are formulated for standard TaqMan® or other probe-based chemistries. These 2X master mixes guarantee outstanding sensitivity and precision for specific target detection, requiring minimal to no protocol optimization.

Benefits

• Reverse transcription and qPCR in one tube with highest sensitivity
• Efficient cDNA synthesis ensured by the thermostable Reverse Transcriptase & advanced RNase Inhibitor blend
• Universal Kit for standard and fast cycling, GC/AT rich templates
• Rapid extension, early Ct values
• Supplied with PCR Water for maximum convenience

Product Range

4X 1-Step RT-qPCR Probe Kits

4X 1-Step RT-qPCR Probe Kits are designed for a sensitive detection of specific RNAs, including viral RNA, in diluted high-volume samples. They combine a robust 4X qPCR mix with a 20X blend of thermostable Reverse Transcriptase and RNase Inhibitor. This formulation allows for a high sample input volume with a reliable outcome of a single step RT-qPCR when working with low copy number samples below 5 copies per reaction. 4X 1-Step RT-qPCR Probe Kits ensure robust performance of both reverse transcription and qPCR reactions, which allows for the highest sensitivity viral RNA detection under fast qPCR cycling conditions.

Benefits

• Robust 4X qPCR mix for high sample volume input up to 10 µl
• Detects <5 RNA copies per reaction
• Reverse transcription and qPCR in one tube
• Ideal for multiplex reactions
• Universal - both standard and fast cycling, GC/AT rich templates

Products

Selection of 1-Step RT-qPCR Solution

Instrument Compatibility for Probe and Dye-based 1-Step RT qPCR Kits