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Immunoassay has undergone different stages, such as radioimmunoassay, fluorescence immunoassay and enzyme-linked immunoassay (ELISA). Chemiluminescence immunoassay (CLIA) is a new stage in the development of immunoassay, which is widely recognized for its environmental protection, rapid and accurate characteristics.

CLIA is a highly sensitive analytical technique for the quantitative detection of multiple trace substances, such as antigens, antibodies, hormones, enzymes, fatty acids, vitamins and drugs, when combined with high specific immune responses.