• Amerigo Scientific Instrument

    Complementary DNA (cDNA) is DNA synthesized from an RNA template. We provide a wide range of tissue cDNAs for the research market, including cDNAs of human healthy and diseased tissue, and cDNAs of mouse, rat, monkey, and plant tissues.

    By modified guanidine thiocyanate techniques, total RNAs are isolated from a variety of tissues. After RNA isolation, the first strand cDNAs are synthesized by MMLV reverse transcriptase with low RNase H activity. Our cDNAs can be directly used as a template for polymerase chain reaction (PCR) or real-time PCR, as well as for gene discovery or gene expression analysis.

    Features

    • Ready to use for PCR
    • Oligo dT primer used to ensure the presence of the entire 3′ end of cDNA
    • 12 kb PCR amplicon successfully obtained with some templates indicating intact cDNAs
    • cDNAs from various tissues of a variety of species
    • Documentation of tissues’ clinical histories available

    Applications

    • Immediate PCR Amplification of known genes
    • Verification of genetic mutation
    • Comparison of a specific gene between different tissues
    • Analysis of mRNA alternative splicing
    • Gene cloning and target sequencing
    Products (385)
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    C1434122
    • Size: 40 rxn
    • Catalog Number: C002096804BIO
    C1434035
    • Size: 40 rxn
    • Catalog Number: C002096803BIO
    C1434010
    • Size: 40 rxn
    • Catalog Number: C002096802BIO
    C1634390
    • Size: 40 rxn
    • Catalog Number: C002096801BIO
    C1634370
    • Size: 40 rxn
    • Catalog Number: C002096800BIO
    C1634360
    • Size: 40 rxn
    • Catalog Number: C002096799BIO
    C1634350
    • Size: 40 rxn
    • Catalog Number: C002096798BIO
    C1634330
    • Size: 40 rxn
    • Catalog Number: C002096796BIO
    C1634340
    • Size: 40 rxn
    • Catalog Number: C002096797BIO
    C1634310
    • Size: 40 rxn
    • Catalog Number: C002096795BIO
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