• Amerigo Scientific Instrument

    Complementary DNA (cDNA) is DNA synthesized from an RNA template. We provide a wide range of tissue cDNAs for the research market, including cDNAs of human healthy and diseased tissue, and cDNAs of mouse, rat, monkey, and plant tissues.

    By modified guanidine thiocyanate techniques, total RNAs are isolated from a variety of tissues. After RNA isolation, the first strand cDNAs are synthesized by MMLV reverse transcriptase with low RNase H activity. Our cDNAs can be directly used as a template for polymerase chain reaction (PCR) or real-time PCR, as well as for gene discovery or gene expression analysis.

    Features

    • Ready to use for PCR
    • Oligo dT primer used to ensure the presence of the entire 3′ end of cDNA
    • 12 kb PCR amplicon successfully obtained with some templates indicating intact cDNAs
    • cDNAs from various tissues of a variety of species
    • Documentation of tissues’ clinical histories available

    Applications

    • Immediate PCR Amplification of known genes
    • Verification of genetic mutation
    • Comparison of a specific gene between different tissues
    • Analysis of mRNA alternative splicing
    • Gene cloning and target sequencing
    Products (385)
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    C1534227-Cy
    • Size: 40 rxn
    • Catalog Number: C002096745BIO
    C1534101-Cy
    • Size: 40 rxn
    • Catalog Number: C002096743BIO
    C1534226-Cy
    • Size: 40 rxn
    • Catalog Number: C002096742BIO
    C1534201-Cy
    • Size: 40 rxn
    • Catalog Number: C002096741BIO
    C1534194-Cy
    • Size: 40 rxn
    • Catalog Number: C002096739BIO
    C1534133-Cy
    • Size: 40 rxn
    • Catalog Number: C002096740BIO
    C1534190-Cy
    • Size: 40 rxn
    • Catalog Number: C002096738BIO
    C1534106-Cy
    • Size: 40 rxn
    • Catalog Number: C002096737BIO
    C1534105-Cy
    • Size: 40 rxn
    • Catalog Number: C002096735BIO
    C1534118-Cy
    • Size: 40 rxn
    • Catalog Number: C002096736BIO
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