Complementary DNA (cDNA) is DNA synthesized from an RNA template. We provide a wide range of tissue cDNAs for the research market, including cDNAs of human healthy and diseased tissue, and cDNAs of mouse, rat, monkey, and plant tissues.
By modified guanidine thiocyanate techniques, total RNAs are isolated from a variety of tissues. After RNA isolation, the first strand cDNAs are synthesized by MMLV reverse transcriptase with low RNase H activity. Our cDNAs can be directly used as a template for polymerase chain reaction (PCR) or real-time PCR, as well as for gene discovery or gene expression analysis.
Features
- Ready to use for PCR
- Oligo dT primer used to ensure the presence of the entire 3′ end of cDNA
- 12 kb PCR amplicon successfully obtained with some templates indicating intact cDNAs
- cDNAs from various tissues of a variety of species
- Documentation of tissues’ clinical histories available
Applications
- Immediate PCR Amplification of known genes
- Verification of genetic mutation
- Comparison of a specific gene between different tissues
- Analysis of mRNA alternative splicing
- Gene cloning and target sequencing
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