Universal cDNA Reverse Transcribed by Oligo dT Primer: Rat Normal Tissues

- Overview
  - Our Rat Universal cDNA is prepared from several male and female Sprague Dawley or Wistar rat whole bodies without fur. This cDNA serves as a standard for comparison of gene expression by RT-PCR or as a gene pool for cloning rat genes
    Our niversal cDNA is prepared using the following method. 10 Âµg total RNA was primed by an oligo dT primer or random hexamer through reverse transcription (RT) using MMLV reverse transcriptase in 40 Âµl final volume. The RT Reaction was stopped by heating at 65Â°C for 10 minutes. The resulting cDNA is contained in 1x RT buffer (1x RT Buffer: 50 mM Tris-CI, pH 8.3, 75 mM KCI, 3 mM MgCI2, 10 mM DTT). The estimated cDNA concentration is 2.5 ng/Âµl. 1 Âµl cDNA is enough for one PCR reaction.
    
    Please contact us at for specific academic pricing.
- Properties
  - Source
    
    Rat
    
    Donor Information
    
    Male: 5-7 wks old, 6 donors / Female: 5-7 wks old, 6 donors
    
    * Research Use Only