-
-
Overview
-
Our Dog Universal cDNA is prepared from major organs of both male and female Beagle dogs. This cDNA serves as a standard for comparison of gene expression by RT-PCR or as a gene pool for cloning dog genes.
Our niversal cDNA is prepared using the following method. 10 µg total RNA was primed by an oligo dT primer or random hexamer through reverse transcription (RT) using MMLV reverse transcriptase in 40 µl final volume. The RT Reaction was stopped by heating at 65°C for 10 minutes. The resulting cDNA is contained in 1x RT buffer (1x RT Buffer: 50 mM Tris-CI, pH 8.3, 75 mM KCI, 3 mM MgCI2, 10 mM DTT). The estimated cDNA concentration is 2.5 ng/µl. 1 µl cDNA is enough for one PCR reaction.Please contact us at for specific academic pricing.
-
- Properties
-
Overview
- Home
- Products
- Life Science
- Analytical Chromatography
- Antibodies
- Assay Kits
- Antigens
- Biochemical Reagents
- Biospecimens
- Cell Culture
- Cell Analysis
- Chemicals
- Cloning and Expression
- Collagen
- Nucleic Acid Detection
- Nucleic Acid Extraction
- Enzymes
- Glycan Analysis
- Hydrogels
- Immunohistochemistry (IHC)
- Ion Exchange Resins
- Liposomes
- Microbiology
- Nanoparticles
- Nucleic Acid Electrophoresis
- Nucleic Acid Synthesis
- PCR
- Proteins
- Protein Biochemistry
- Protein Gel Electrophoresis
- Transfection Reagents
- Ubiquitin-Proteasome
- Venoms
- Lab Solutions
- Amerigo Scientific Instrument
- 3D Bioprinting
- Medical Devices
- Life Science
- Services
- About Us
- Contact Us
.
