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Universal cDNA Reverse Transcribed by Oligo dT Primer: Monkey Normal Tissues

Universal cDNA Reverse Transcribed by Oligo dT Primer: Monkey Normal Tissues

Catalog Number:
C002096829BIO
Mfr. No.:
C4534565
Price:
$488
  • Size:
    100 rxn
    Quantity:
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      • Overview

        • Our Monkey Universal cDNA is prepared from major organs of both male and female Cynomolgus and Rhesus monkeys. This cDNA serves as a standard for comparison of gene expression by RT-PCR or as a gene pool for cloning monkey genes
          Our niversal cDNA is prepared using the following method. 10 µg total RNA was primed by an oligo dT primer or random hexamer through reverse transcription (RT) using MMLV reverse transcriptase in 40 µl final volume. The RT Reaction was stopped by heating at 65°C for 10 minutes. The resulting cDNA is contained in 1x RT buffer (1x RT Buffer: 50 mM Tris-CI, pH 8.3, 75 mM KCI, 3 mM MgCI2, 10 mM DTT). The estimated cDNA concentration is 2.5 ng/µl. 1 µl cDNA is enough for one PCR reaction.

          Please contact us at for specific academic pricing.

      • Properties
        • Source
          Monkey
          Donor Information
          Male: 4.5-5 yrs old, 2 donors / Female: 3.5-4.5 yrs old, 3 donors

          * Research Use Only

    Datasheet

    Documents

    Certificate of Analysis

    Data Sheet

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