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Universal cDNA Reverse Transcribed by Random Hexamer: Mouse Normal Tissues

Universal cDNA Reverse Transcribed by Random Hexamer: Mouse Normal Tissues

Catalog Number:
C002096832BIO
Mfr. No.:
C4334566-R
Price:
$488
  • Size:
    100 rxn
    Quantity:
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      • Overview

        • Our Mouse Universal cDNA is prepared from several male and female Balb/C mice whole bodies without fur. This cDNA serves as a standard for comparison of gene expression by RT-PCR or as a gene pool for cloning mouse genes
          Our niversal cDNA is prepared using the following method. 10 µg total RNA was primed by an oligo dT primer or random hexamer through reverse transcription (RT) using MMLV reverse transcriptase in 40 µl final volume. The RT Reaction was stopped by heating at 65°C for 10 minutes. The resulting cDNA is contained in 1x RT buffer (1x RT Buffer: 50 mM Tris-CI, pH 8.3, 75 mM KCI, 3 mM MgCI2, 10 mM DTT). The estimated cDNA concentration is 2.5 ng/µl. 1 µl cDNA is enough for one PCR reaction.

          Please contact us at for specific academic pricing.

      • Properties
        • Source
          Mouse
          Donor Information
          Male: 5-6 wks old, 5 donors / Female: 5-6 wks old, 16 donors

          * Research Use Only

    Datasheet

    Documents

    Certificate of Analysis

    Data Sheet

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