Universal cDNA Reverse Transcribed by Random Hexamer: Human Normal Tissues

Our Human Universal cDNA is prepared from major organs of both male and female normal adult donors. This cDNA is ready to use for comparison of gene expression by RT-PCR or as a gene pool for cloning human genes. It is a pool from 2 male donors (64 and 71 years old) and 3 female donors (32, 69, and 76 years old).
Our niversal cDNA is prepared using the following method. 10 µg total RNA was primed by an oligo dT primer or random hexamer through reverse transcription (RT) using MMLV reverse transcriptase in 40 µl final volume. The RT Reaction was stopped by heating at 65°C for 10 minutes. The resulting cDNA is contained in 1x RT buffer (1x RT Buffer: 50 mM Tris-CI, pH 8.3, 75 mM KCI, 3 mM MgCI2, 10 mM DTT). The estimated cDNA concentration is 2.5 ng/µl. 1 µl cDNA is enough for one PCR reaction.

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