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Overview
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Iron is the most abundant transition metal element within organisms, and it participates in various physiological activities. Recently, free iron in living cells has attracted attention because its high reactivity may be related to cellular damage or death. Free iron exists in its stable redox states, namely ferrous ion (Fe2+) and ferric ion (Fe3+). In living cells, understanding the behavior of Fe2+is considered more important than understanding that of Fe3+ because of the intracellular reductive environment, metal transporters, and the water solubility of Fe2+. FerroOrange is a novel fluorescent probe that enables live-cell fluorescent imaging of intracellular Fe2+.
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Overview