GSSG/GSH Quantification Kit

- Overview
  - Glutathione (γ-L-glutamyl-L-cysteinylglycine) is a tripeptide present in the body, and it is involved in antioxidation, drug metabolism, and other as enzyme substrate of glutathione peroxidase, glutathione S-transferase, and thiol transferase, etc. Glutathione is usually present as reduced form (GSH), but GSH is converted into its oxidized form (GSSG) by stimulation such as oxidative stress. Therefore, the ratio of GSH and GSSG has been noted as index of oxidative stress.
    The GSSG/GSH Quantification kit contains Masking Reagent of GSH. The GSH can be deactivated in the sample by simply adding the Masking Reagent. Therefore, only the GSSG is detected by measuring the absorption (λmax = 412 nm) of DTNB (5,5’-dithiobis (2-nitrobenzoic acid) using the enzymatic recycling system. Also, GSH can be determined the quantity by subtracting GSSG from the total amount of glutathione.
    The kit is limited to quantifying GSH/GSSG concentration from 0.5 μmol/l to 50 μmol/l and GSSG concentration from 0.5 μmol/l to 25 μmol/l.
    
    Please contact us at for specific academic pricing.
    
    More Details
    
    Cellular Metabolism and Anticancer Effects
    
    Polyol Metabolism and Hyperglycemic State
    
    Cellular Senescence and Mitochondrial Disfunction Due to Lipid Accumulation
    
    Ferroptosis
- Properties
  - Categories
    
    SOD Activity, Glutathione, DNA Damage, Antioxidant capacity
    
    Storage
    
    0-5°C, protect from light
    
    Shipping
    
    ambient temperature
    
    * For research use only
- Applications
  - Application
    
    GSSG/GSH Ratio
- Reference
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    4) S. A. McGrath-Morrow and J. Stahl, "Inhibition of Glutamine Synthetase in A549 Cells During Hyperoxia", Am. J. Respir. Cell Mol. Biol., 2002, 27, 99.
    
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    8) K. Okabayashi, T. Narita, Y. Takahashi and H. Sugiya, "Effrct of Oxidative Stress on Secretory Function in Salivary Gland Cells", Oxidative Stress-Environmental Induction and Dietary Antioxidants, V. Lushchak, InTech, 2012, 189.
    
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    11) M. Sueyoshi, M. Fukunaga, M. Mei, A. Nakajima, G. Tanaka, T. Murase, Y. Narita, S. Hirata, and D. Kadowaki, "Effects of lactulose on renal function and gut microbiota in adenine-induced chronic kidney disease rats", Clinical and Experimental Nephrology., 2019,doi: 10.1007/s10157-019-01727-4.
    
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