GSSG/GSH Quantification Kit

Name: GSSG/GSH Quantification Kit
Price: 912.00 USD

Catalog Number:

CFA1108132DOJ

Mfr. No.:

G257-10

Price:

$912

Size:

200 tests

Quantity:

Add to Cart:

Add to Cart

- Overview
  - Glutathione (γ-L-glutamyl-L-cysteinylglycine) is a tripeptide present in the body, and it is involved in antioxidation, drug metabolism, and other as enzyme substrate of glutathione peroxidase, glutathione S-transferase, and thiol transferase, etc. Glutathione is usually present as reduced form (GSH), but GSH is converted into its oxidized form (GSSG) by stimulation such as oxidative stress. Therefore, the ratio of GSH and GSSG has been noted as index of oxidative stress.
    The GSSG/GSH Quantification kit contains Masking Reagent of GSH. The GSH can be deactivated in the sample by simply adding the Masking Reagent. Therefore, only the GSSG is detected by measuring the absorption (λmax = 412 nm) of DTNB (5,5’-dithiobis (2-nitrobenzoic acid) using the enzymatic recycling system. Also, GSH can be determined the quantity by subtracting GSSG from the total amount of glutathione.
    The kit is limited to quantifying GSH/GSSG concentration from 0.5 μmol/l to 50 μmol/l and GSSG concentration from 0.5 μmol/l to 25 μmol/l.
    
    Please contact us at for specific academic pricing.
    
    More Details
    
    Cellular Metabolism and Anticancer Effects
    
    Polyol Metabolism and Hyperglycemic State
    
    Cellular Senescence and Mitochondrial Disfunction Due to Lipid Accumulation
    
    Ferroptosis
- Properties
  - Categories
    
    SOD Activity, Glutathione, DNA Damage, Antioxidant capacity
    
    Storage
    
    0-5°C, protect from light
    
    Shipping
    
    ambient temperature
    
    * For research use only
- Applications
  - Application
    
    GSSG/GSH Ratio
- Reference
  - 1) M. E. Anderson, "Determination of Glutathione and Glutathione Disulfide in Biological Samples", Methods in Enzymol., 1985, 113, 548.
    
    2) M. A. Baker, G. J. Cerniglia and A. Zaman, "Microtiter Plate Assay for the Measurement of Glutathione and Glutathione Disulfide in Large Numbers of Biological Samples", Anal. Biochem., 1990, 190, 360.
    
    3) C. Vandeputte, I. Guizon, I. Genestie-Denis, B. Vannier and G. Lorenzon, "A Micrototer Plate Assay for Total Glutathione and Glutathione Disulfide Contents in Cultured/isolated Cells: Performance Study of a New Miniaturized Protocol", Cell Biol. Toxicol., 1994, 10, 415.
    
    4) S. A. McGrath-Morrow and J. Stahl, "Inhibition of Glutamine Synthetase in A549 Cells During Hyperoxia", Am. J. Respir. Cell Mol. Biol., 2002, 27, 99.
    
    5) T. Sato, K. Seyama, Y. Sato, H. Mori, S. Souma, T. Akiyoshi, Y. Kodama, T. Mori, S. Goto, K. Takahashi, Y. Fukuchi, N. Maruyama and A. Ishigami, "Senescence Marker Protein-30 Protects Mice Lungs from Oxidative Stress, Aging, and Smoking", Am. J. Respir. Crit. Care Med., 2006, 174, 530.
    
    6) M. L. Mulhern, C. J. Madson, A. Danford, K. Ikesugi, P. F. Kador and T. Shinohara, "The Unfolded Protein Response in Lens Epithelial Cells from Galactosemic Rat Lenses", Invest. Ophthalmol. Vis. Sci., 2006, 47(9), 3951.
    
    7) N. Miura, Y. Yanagiba, K. Ohtani, M. Mita, and M. Togawa, "Diurnal Variation of Cadmium-induced Mortality in Mice", J. Toxicol. Sci., 2012, 37(1), 191.
    
    8) K. Okabayashi, T. Narita, Y. Takahashi and H. Sugiya, "Effrct of Oxidative Stress on Secretory Function in Salivary Gland Cells", Oxidative Stress-Environmental Induction and Dietary Antioxidants, V. Lushchak, InTech, 2012, 189.
    
    9) G. Tian, J. Sawashita, H. Kubo, S. Nishio, S. Hashimoto, N. Suzuki, H. Yoshimura, M. Tsuruoka, Y. Wang, Y. Liu, H. Luo, Z. Xu, M. Mori, M. Kitano, K. Hosoe, T. Takeda, S. Usami and K. Higuchi, "Ubiquinol-10 Supplementation Activates Mitochondria Functions to Decelerate Senescence in Senescence-Accelerated Mice", Antioxid. Redox Signal., 2014, 20(16), 2606.
    
    10) H. Nakagawa, A. Umemura, K. Taniguchi, J. Font-Burgada, D. Dhar, H. Ogata, Z. Zhong, M. A. Valasek, E. Seki, J. Hidalgo, K. Koike, R. J. Kaufman and M. Karin, "ER Stress Cooperates with Hypernutrition to Trigger TNF-Dependent Spontaneous HCC Development", Cancer cell, 2014, 26(3), 331.
    
    11) M. Sueyoshi, M. Fukunaga, M. Mei, A. Nakajima, G. Tanaka, T. Murase, Y. Narita, S. Hirata, and D. Kadowaki, "Effects of lactulose on renal function and gut microbiota in adenine-induced chronic kidney disease rats", Clinical and Experimental Nephrology., 2019,doi: 10.1007/s10157-019-01727-4.
    
    12) S. Shiromizu, T. Yamauchi, N. Kusunose, N. Matsunaga, S. Koyanagi and S. Ohdo, "Dosing Time-Dependent Changes in the Anti-tumor Effect of xCT Inhibitor Erastin in Human Breast Cancer Xenograft Mice", Biol. Pharm. Bull.., 2019, 42, (11), 1921-1925.