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Overview
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All-Fect is a highly effective, broadly-acting gene delivery system useful in suspension cell transfection and It has been tailored as a siRNA pDNA cotransfection reagent for a variety of cell lines and is capable of undergoing multivalent interactions with polynucleotides and encapsulating co-incubated polynucleotides into 100-200 nm particles with a net positive charge. The complexation between the polynucleotides and the Al-Fect occurs in aqueous buffers, obviating the need for organic solvents during preparation. All-Fect is a non-integrating carrier, so that the genetic make-up of host cells is not altered after treatment with the transfection reagent making it an excellent choice for transient transfection applications. All-Fect has been tested and found effective in different types of attachment dependent cells as well as suspension cells. As with all transfection reagents, formulations of All-Fect with DNA or RNA may need to be optimized for specific cell types and transfection conditions.
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More Details
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- Properties
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Overview