CRISP-Fect RNP Transfection Reagent

The CRISP-Fect reagent is a highly effective transfection reagent optimized for ribonucleoprotein (RNP) delivery to both attachment-dependent and suspension-growing cells. CRISP-Fect is able to bind RNPs complexes of Cas9 endonuclease and sgRNA. Upon co-incubation, the transfection reagent forms 50-150 nm particles with RNPs that bear a net positive charge. The formation of complexes is performed in aqueous buffers without any organic solvents during preparation. CRISP-Fect is a non-integrating reagent and does not cause genetic changes on its own after treatment. CRISP-Fect has been confirmed to provide effective delivery of Cas9 RNPs in different cell types. Figure 1 shows typical delivery of RNPs to attachment-dependent cells (breast cancer cells) and suspension-growing cells (Jurkat T-cells). As with all transfection reagents, formulations of CRISP-Fect with the intended cargo may need to be optimized for specific cell types and transfection conditions.

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