iPSC Cryopreservation Medium - GMP grade

The cryopreservation solution product for induced pluripotent stem cells (iPSC) can achieve good long-term cryopreservation after cryopreservation with this product. After 3 months of cryopreservation at -196°C, the iPSC cell clusters were well attached to the wall after recovery. After 24h, 48h, and 72h of observation, it was found that the clone edge was clear, the interior was dense, and there was no natural differentiation phenomenon overall.
After 72 hours of recovery, the iPSC stained with alkaline phosphatase showed alkaline phosphatase positive.
72 hours after recovery, the iPSC expressed OCT3/4, Nanog, TRA-1-60, and SSEA4, proving that the cells maintained a good pluripotency state after recovery, and the proportion of undifferentiated cells was high.

Features
· Developed based on controlled ice freezing technology
· Applicable to cell cluster and single cell freezing system
· Double cryoprotectant, containing self-developed controlled ice protectant + 10% USP grade DMSO
· No serum, no protein, no animal-derived ingredients
· Full pharmacopoeia ingredients, clear components, pharmaceutical grade raw materials
· Sterile, low endotoxin, <0.125 EU/mL
· High safety, high recovery rate, ultra-high ease of use
· GMP grade
· Ready-to-use
· Easy to store and transport, can be stored at 2-8°C and transported over long distances
· Support direct freezing, no programmed cooling required (Short-term storage: -80°C freezer. For long-term storage, transfer to -196°C liquid nitrogen after 12 hours.)

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