Human epidermal transglutaminase

- Overview
  - His6-rhTG3 is based on the TGM3-allele from clone DKFZp686J0716 (isolated from different tissues), corrected by the insertion of the missing T at Position 435. It is N-terminally fused to a hexahistidine-tag resulting in the encoded N-terminal amino acid sequence MHHHHHHAALGV… . His6-rhTG3 is produced in insect cells and purified by ion metal chelating and ion exchange chromatography to more than 95 % purity.
    
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    Background
    
    This product replaces product replaces product number T124.
    
    More Details
    
    Zedira
    
    Gluten-related Disorder Products
- Properties
  - Name
    
    TG3, TGase 3, epidermal protein-glutamine-γ-glutamyltransferase, TGE
    
    Source
    
    Insect cells
    
    Type
    
    Recombinant Proteins
    
    Formulation
    
    The Transglutaminase is lyophilized from 20 mM Tris-HCl pH 7.5, 150 mM NaCl, 1 mM EDTA and 1 mM DTT. Sample contains maltodextrin.
    
    Storage
    
    Storage for several months is possible at ≤ - 20°C. Delivery is possible at ambient temperature
    
    Note
    
    INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS. This product replaces TG3, recombinantly produced in E. coli (T012).
    
    Molecular Weight
    
    78 kDa
    
    Class
    
    Transglutaminase 3
    
    Reconstitution
    
    Add the volume of water specified in the certificate of analysis under aliquotation to the vial of lyophilized powder. Rotate vial gently until solid dissolves. After reconstitution the solution should be stored frozen in working aliquots. For short term storage keep cooled on ice.
    
    Activity
    
    His6-rhTG3 is a Ca2+-dependent enzyme.
    10 μl His6-rhTG3 (1 μg/μl)
    35 μl 50 mM Tris-HCl pH 8
    5 μl Dispase I (Roche) (0.2 μg/μl)
    Incubate for 20 min at 30°C
    Add 10 mM Ca2+ when measuring His6-rhTG3 activity.
- Applications
  - Application Description
    
    His6-rhTG3 catalyzes acyl transfer reactions from glutamine residues in proteins or peptides to primary amines, e. g. the formation of ε-(γ-glutamyl) lysine bonds between proteins by transferring the acyl group of a peptide-bound glutamine residue to the primary amino group of a peptide-bound lysine residue. His6-rhTG3 may also be used for immunoprecipitation and the detection of TG3 autoantibodies.
- Reference
  - Hietikko et al., Acta. Derm. Venereol. 2018, 98:366-72
    Yamane et al., FEBS J. 2010, 277:3564-74