Phenyldiazirine sxLink™ (T2A14)

Phenyldiazirine sxLink™ (T2A14) is a proprietary photo-crosslinking reagent developed for studying biomolecular interactions.

This reagent combines the highly efficient carbene-generating phenyldiazirine group with a cleavable disulfide crosslinking group via a super hydrophilic AqueaTether™ (AqT™) based linker. These structural features make it highly advantageous to use this reagent over traditional photo-crosslinking reagents.

1. Trifluoromethyl phenyldiazirine (TFMP) group: TFMP photolyzes around 360 nm, at which photodamage to biomolecules is minimized. The generated carbene inserts C–H bonds into the neighboring biomolecular partner within picoseconds. Because the electron-withdrawing trifluoromethyl group confers stability on the intermediate diazo-isomer, no side products are detected under normal labeling conditions.
2. Reversible disulfide for chemical crosslinking of any biomolecule containing a free thiol.
3. Hydrophilic AqT™ linkers: TFMP group is highly hydrophobic, biomolecules labeled with a TFMP using a traditional ethylene and ethylene glycol-type linker tend to aggregate and destabilize the labeled protein. T2A14 AqT™ linker is made by linking two threitols together, greatly increasing the hydrophilicity of sxLink™ and its water solubility (2.2 mg/mL saturated solution). The molecule is also more biocompatible with decreased non-specific hydrophobic interactions with other biomolecules, allowing high loading of phenyldiazirine groups.
The product is sold as a lyophilized powder in either 1 vial of 250 µg (Cat# CM81401-250UG) or 4 vials of 250 µg (Cat# CM81401-4x250UG). One vial of dissolution buffer is included in each configuration.

Key Features of the Product
• More hydrophilic than traditional photo-crosslinking reagent, soluble, and biocompatible
• High loading with minimized aggregation
• Reversible linkage
• Contains an efficient carbene-generating photo-crosslinking group

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