AqT™ Biotin Active Ester Antibody Labeling Kit (T2A15)

AqT™ Biotin Active Ester Antibody Labeling kit (T2A15) is a proprietary biotinylation kit developed for obtaining high quality and high loading of biotinylated antibody with minimal or no aggregation. The kit uses a biotin modified with a super-hydrophilic, water-soluble, and charge neutral AqueaTether™ (AqT™) T2A15 linker to improve its water solubility and decrease its non-specific interaction with other proteins or plastic surfaces. Biotinylated antibodies with AqT™ linker can largely retain its native properties. AqT™ T2A15 linker composed of two threitols and 15 atom lengths. This atom length is equivalent to PEG4 (16 atom lengths) and, based on our experience, can largely keep the bivalent molecule function independent. The conjugates we have tried with this atom length include antibody oligo, streptavidin oligo, and HRP oligo, among others.
The CM86140 kit is designed to biotinylate any antibody via surface amines of the antibody. The user supplies the antibody. Using the kit components, the user biotinylates their antibody in one simple step, followed by filtration-type purification to remove any unreacted AqT™-biotin, exchanging it with PBS or other buffer for downstream application. A quick 10-minute biotin assay (protocol and reagent) is also included in the kit to obtain the biotinylation level immediately after labeling. The biotinylation level can easily be tuned by increasing or decreasing the reaction temperature. The hands-on time is less than 30 minutes, and the whole preparation time varies from 3 h to overnight depending on the reaction time.

The kit has multiple configurations for labeling various amounts of antibody (10 μg to 3 mg) with either one (x1) or three (x3) antibody samples.

• Requirements for antibody:
MW: ≥ 100 KDa (if MW is lower, please use an alternative kit)
Purity: preferably > 90% pure by gel electrophoresis
Amount: 10 μg to 3 mg protein content as measured by UV

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