Rat Apoptosis Real-time PCR arrays

We offer SignArrays® real-time PCR arrays for highly specific, reliable, and reproducible results for signaling pathways analysis, even with small amounts of precious samples like FFPE or LCM samples.

qPCR arrays SignArrays® characteristics:
ALL IN ONE SOLUTION
▪ Specific signaling pathway (96-well or 384-well plates)
▪ Sensitive and highly accurate qPCR reagents (Perfect Master Mix SYBR® Green & EvaGreen)
▪ Free data analysis software
▪ Compatible with all qPCR instruments
STRICT INTRON FLANKING PRIMER DESIGN
▪ In order to guarantee sensitive, specific, and accurate results, every primer set is designed with highly stringent criteria and validated at the experimental level on a large collection of tissues and cell lines on our high-throughput molecular platform thanks to strict experimental quality controls.
▪ We offer you very high-quality primers.
▪ With a primer design on 2 exons to guarantee high specificity in qPCR and avoid any contaminating gDNA amplification.
A FAST AND EASY PROTOCOL
▪ Signaling pathway SignArrays® results in less than 2 hours!
▪ SignArrays® protocols has been standardized to allow qPCR analysis in the easiest way.
▪ Just prepare the qPCR mixes of the Perfect Master Mix SYBR® Green and cDNA samples, and dispense.

Please contact us at for specific academic pricing.

Background

SignArrays® real-time PCR arrays in 96-well or 384-well plate formats are compatible with most of all qPCR instruments on the market.
Each SignArrays® 96 allows to analyze 84 genes of interest, specific to a signaling or pathological pathway with 8 reference genes necessary to the robust normalization step of your qPCR data and 4 quality controls.
All signaling pathways are validated at the experimental level on a large collection of tissues and cell lines on a high-throughput molecular platform, thanks to stringent and strong quality control criteria to guarantee you the best results.

Available for many species: Homo sapiens, Mus musculus, Rattus norvegicus, Sus scrofa and more on demand!

Apoptosis or programmed cell death is a highly regulated process critical for normal development and tissue homeostasis. Aberrant regulation of apoptosis can lead to cancer. Apoptosis is induced from signals inside or outside the cell including radiation, viral infection, growth factors, and hormones. Apoptosis involves signature morphological changes induced by caspases, which are activated upon induction of apoptotic signalling and cleave downstream molecules to facilitate the apoptotic cascade. The induction of apoptosis can occur through two pathways: the intrinsic apoptotic pathway which involves signalling through the mitochondria and the extrinsic apoptotic pathway which is initiated through activation of cell surface death receptors. Apoptotic signalling through the intrinsic pathway primarily involves activation of the proapoptotic Bcl-2 family members Bax and Bak, which facilitate release of cytochome C from the mitochondria and subsequent caspase-9 cleavage or activation. The activated caspase-9 will finally cleave or activate the downstream effector caspases such as caspase-3 and -7, leading to apoptosis.This pathway is negatively regulated by several antiapoptotic Bcl-2 family members such as Bcl-2 and Bcl-XL. Apoptotic signalling through the extrinsic pathway is initiated by ligand binding to death receptors or by induction of trimerization of the receptors. The death receptors belong to the tumor necrosis factor (TNF) receptor superfamily, which includes Fas, TNFR1, DR3, DR4 (TRAIL-R1),DR5(TRAIL-R2),and DR6.