pColiExpress™ I LIC Cloning & Expression Kit is a highly efficient, versatile and fast system of DNA cloning vectors for protein expression in E. coli. All family systems are based in directional ligase-independent-cloning (LIC) technology, a rapid procedure that provides high-cloning efficiencies and the fast production of a large quantity of any desired protein.
Advantages & Features
Highly efficient cloning system.
Special design that allows the cell to house larger numbers of copies than other plasmids with ori pBR322.
Linearized vector: ready for ligation with your PCR amplified with the recommended primers.
Minimum background: lower than 1%.
Time-saving protocol: avoids any step required after PCR.
Higher protein expression than other suppliers.
Cost avoidance: avoids the use of expensive phosphorylated primers.
Versatility: cloning of PCR fragments amplified with any type of DNA Polymerase.
Risk-free: product covered by our Quality 100% Guarantee.
Please contact us at for specific academic pricing.
CategoriesBacterial ExpressionStorage-20 °C (NON Frost-Free Freezer). 24 months from the date of purchase, if it is properly stored.ShippingGel pack.
* This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals.
Application DescriptionFor a highly Efficient & Versatile Protein Expression (LIC technology based) in E. coli from PCR fragment cloned.
Cloning of PCR fragments for subsequent expression of proteins in E. coli.
Cloning of PCR fragments with Glue Enzyme.
Expression of proteins under the control of the T7 promoter.
Protein expression in BL21 (DE3) or BL21 (DE3) (pLys).