BE-DOUBLEFLOW STANDARD

- Overview
  - BE-Doubleflow: is our most advanced device. It consists of two perfusable channels connected via a porous membrane. Explore the crosstalk between different 2D and 3D cultures in a biomimetic environment and control the efficiency of the interaction by selecting the optimal pore size for your application. This is the optimal device when a hypoxia environment is needed, for studying the effect of circulating particles (bacteria, immune system, circulating tumor cells) and for endothelium/epithelium barrier when no ALI is needed or when flux plays a role in both sides of the coculture.
    
    Culture Models
    
    • Explore the crosstalk in the endothelium epithelium barrier under flow in both channels of the device, mimicking a more physiological environment for the study of different organs such as kidney, liver, gut or heart, among others.
    
    • Combine 3D and 2D cultures in a hypoxia environment where oxygen is supplied to the cells via the medium flowing in one of the channels.
    
    • Research the effect of circulating particles such as circulating tumor cells, immune system cells, bacteria, fungi, viruses and many more in a single cell culture, or in a complex coculture.
    
    Device features
    • Easy to use: Be-Doubleflow is compatible with any type of optical microscopy and its slide format has been chosen for easy handling under a microscope.
    • Easy to connect: Be-Doubleflow is compatible with all microfluidic flow control systems (syringe, peristaltic pumps, pressure control systems, rocker systems…).
    • No unspecific absorption: Unlike in other PDMS devices, Be -Doubleflow is made of lipophobic thermoplastic materials and does not present unspecific drug absorption issues. It allows inmunohistochemistry with fluorescent detection.
    • Cell recovery: The cell cultures used in the Be-Doubleflow can be easily recover for further experimentation.
    
    Please contact us at for specific academic pricing.
    
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    Microfluidic Chips
- Properties
  - Categories
    
    Microfluidic Chips
- Applications
  - Application Description
    
    BE-Doubleflow allows endothelium/epithelium barrier coculture where a hypoxia environment is needed or when flux plays a role also in the epithelium culture (Kidney, liver, heart, lung, gut…). In addition, its two perfusable channels offer a perfect environment for studying the effect of circulating particles (bacteria, immune response, circulating tumor cells).
    Kidney on chip: The coculture of HPTC cells and vascular endothelial cells clearly improves performance of the HPTCs and increases the maintenance of the renal epithelia in vitro. Using BE-Doubleflow it is possible to coculture both type of cells simultaneously, circulating urine on the renal epithelium culture and blood like medium in the vascular culture.
- Reference
  - 1.De Miguel, D. et al. TRAIL-coated lipid-nanoparticles overcome resistance to soluble recombinant TRAIL in non-small cell lung cancer cells. Nanotechnology 27, 185101 (2016). 2.Ayuso, J. M. et al. SU-8 Based Microdevices to Study Self-Induced Chemotaxis in 3D Microenvironments. Front. Mater. 2, 1–10 (2015). 3.De Miguel, D. et al. TRAIL-coated lipid-nanoparticles overcome resistance to soluble recombinant TRAIL in non-small cell lung cancer cells. Nanotechnology 27, 185101 (2016). 4.De Miguel, D. et al. Improved Anti-Tumor Activity of Novel Highly Bioactive Liposome-Bound TRAIL in Breast Cancer Cells. Recent Pat. Anticancer. Drug Discov. 11, 197–214 (2016). 5.De Miguel, D. et al. High-order TRAIL oligomer formation in TRAIL-coated lipid nanoparticles enhances DR5 cross-linking and increases antitumour effect against colon cancer. Cancer Lett. 383, 250–260 (2016). 6.Ayuso, J. M. et al. Development and characterization of a microfluidic model of the tumour microenvironment. Sci. Rep. 6, 36086 (2016). 7.Martínez-gonzález, A. et al. Systems Biology of Tumor Microenvironment. vol. 936 (Springer International Publishing, 2016). 8.Ayuso, J. M. et al. Glioblastoma on a microfluidic chip: Generating pseudopalisades and enhancing aggressiveness through blood vessel obstruction events. Neuro. Oncol. 19, now230 (2017). 9.Virumbrales-Muñoz, M. et al. Enabling cell recovery from 3D cell culture microfluidic devices for tumour microenvironment biomarker profiling. Sci. Rep. 9, (2019).

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User Guide

BE-DOUBLEFLOW STANDARD

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