BE-DOUBLEFLOW STANDARD

Name: BE-DOUBLEFLOW STANDARD
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CCS1095956BEO

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BE-DOUBLEFLOW

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10 devices per box

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Overview

BE-Doubleflow: is our most advanced device. It consists of two perfusable channels connected via a porous membrane. Explore the crosstalk between different 2D and 3D cultures in a biomimetic environment and control the efficiency of the interaction by selecting the optimal pore size for your application. This is the optimal device when a hypoxia environment is needed, for studying the effect of circulating particles (bacteria, immune system, circulating tumor cells) and for endothelium/epithelium barrier when no ALI is needed or when flux plays a role in both sides of the coculture.

Culture Models

• Explore the crosstalk in the endothelium epithelium barrier under flow in both channels of the device, mimicking a more physiological environment for the study of different organs such as kidney, liver, gut or heart, among others.

• Combine 3D and 2D cultures in a hypoxia environment where oxygen is supplied to the cells via the medium flowing in one of the channels.

• Research the effect of circulating particles such as circulating tumor cells, immune system cells, bacteria, fungi, viruses and many more in a single cell culture, or in a complex coculture.

Device features
• Easy to use: Be-Doubleflow is compatible with any type of optical microscopy and its slide format has been chosen for easy handling under a microscope.
• Easy to connect: Be-Doubleflow is compatible with all microfluidic flow control systems (syringe, peristaltic pumps, pressure control systems, rocker systems…).
• No unspecific absorption: Unlike in other PDMS devices, Be -Doubleflow is made of lipophobic thermoplastic materials and does not present unspecific drug absorption issues. It allows inmunohistochemistry with fluorescent detection.
• Cell recovery: The cell cultures used in the Be-Doubleflow can be easily recover for further experimentation.

Please contact us at for specific academic pricing.

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Microfluidic Chips

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Microfluidic Chips

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Application Description

BE-Doubleflow allows endothelium/epithelium barrier coculture where a hypoxia environment is needed or when flux plays a role also in the epithelium culture (Kidney, liver, heart, lung, gut…). In addition, its two perfusable channels offer a perfect environment for studying the effect of circulating particles (bacteria, immune response, circulating tumor cells).
Kidney on chip: The coculture of HPTC cells and vascular endothelial cells clearly improves performance of the HPTCs and increases the maintenance of the renal epithelia in vitro. Using BE-Doubleflow it is possible to coculture both type of cells simultaneously, circulating urine on the renal epithelium culture and blood like medium in the vascular culture.

Reference

1.De Miguel, D. et al. TRAIL-coated lipid-nanoparticles overcome resistance to soluble recombinant TRAIL in non-small cell lung cancer cells. Nanotechnology 27, 185101 (2016). 2.Ayuso, J. M. et al. SU-8 Based Microdevices to Study Self-Induced Chemotaxis in 3D Microenvironments. Front. Mater. 2, 1–10 (2015). 3.De Miguel, D. et al. TRAIL-coated lipid-nanoparticles overcome resistance to soluble recombinant TRAIL in non-small cell lung cancer cells. Nanotechnology 27, 185101 (2016). 4.De Miguel, D. et al. Improved Anti-Tumor Activity of Novel Highly Bioactive Liposome-Bound TRAIL in Breast Cancer Cells. Recent Pat. Anticancer. Drug Discov. 11, 197–214 (2016). 5.De Miguel, D. et al. High-order TRAIL oligomer formation in TRAIL-coated lipid nanoparticles enhances DR5 cross-linking and increases antitumour effect against colon cancer. Cancer Lett. 383, 250–260 (2016). 6.Ayuso, J. M. et al. Development and characterization of a microfluidic model of the tumour microenvironment. Sci. Rep. 6, 36086 (2016). 7.Martínez-gonzález, A. et al. Systems Biology of Tumor Microenvironment. vol. 936 (Springer International Publishing, 2016). 8.Ayuso, J. M. et al. Glioblastoma on a microfluidic chip: Generating pseudopalisades and enhancing aggressiveness through blood vessel obstruction events. Neuro. Oncol. 19, now230 (2017). 9.Virumbrales-Muñoz, M. et al. Enabling cell recovery from 3D cell culture microfluidic devices for tumour microenvironment biomarker profiling. Sci. Rep. 9, (2019).

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