Custom RNA Oligo Synthesis

RNA oligonucleotides (oligos) are short single-stranded or double-stranded RNA sequences that have been widely used in biological research, drug development, and diagnostics due to their unique structures and functions. Common types of RNA oligos include antisense oligonucleotides (ASOs), small interfering RNA (siRNA), microRNA (miRNA), aptamers, and ribozymes. ASOs are synthetic molecules designed to complementarily bind specific mRNA sequences, forming RNA-DNA hybrids to block the mRNA translation and inhibit the expression of target proteins. siRNA is a double-stranded RNA molecule that triggers the RNA interference pathway to specifically degrade complementary mRNA, achieving gene silencing. MicroRNA is a endogenous small non-coding RNA molecule that regulates gene expression by binding to the 3' untranslated region (3'UTR) of the target mRNA. Therapeutics based on these functional RNA oligos are being developed to treat various diseases, particularly cancers, viral infections, and genetic disorders. In addition, synthetic RNA oligos can serve as hybridization probes for detecting specific RNA sequences or as reverse transcription primers for initiating complementary DNA synthesis from RNA templates.

In the CRISPR-Cas system, CRISPR RNA (crRNA) and single guide RNA (sgRNA) are two critical RNA molecules for directing Cas proteins to target DNA sequences. crRNA is a short RNA containing a guide sequence complementary to the target DNA, which must interact with trans-activating CRISPR RNA (tracrRNA) to form a duplex structure for recognition by the Cas9 protein and subsequent DNA cleavage. sgRNA is an engineered chimeric RNA that combines the crRNA guide sequence with the tracrRNA scaffold into a single RNA strand. This innovation significantly simplifies CRISPR-Cas9 applications, requiring only sgRNA and Cas9 protein for gene editing. sgRNA is widely employed in CRISPR-based gene editing technologies for gene knockout, knock-in, and repair. sgRNA libraries further enable large-scale genetic screening.

RNA oligos can be chemically modified to enhance stability and delivery specificity. Amerigo Scientific provides high-quality, cost-effective custom RNA oligos with diverse types and specific modifications to meet different needs in advancing gene editing, germplasm transformation, and genetic regulation. All RNA oligos are synthesized using premium raw materials and processes, purified via OPC or HPLC methods, and subjected to 100% mass spectrometry analysis to ensure product quality.

Advantages of Our RNA Oligo Synthesis

Amerigo Scientific can synthesize RNA oligonucleotides up to 180 nucleotides in length, and the types of customized RNA oligonucleotides include sgRNA, siRNA, miRNA, ASO, and various modified RNAs. Based on synthesis techniques with high coupling efficiency between bases, high product stability, and low cost, we provide high-quality, cost-effective RNA oligo synthesis services with short turnaround times.

• Synthesis services for various RNA products such as sgRNA, siRNA, miRNA, and ASO
• Rapid synthesis cycle, and regular orders completed within 3-4 days
• 100% mass spectrometry with a variety of additional detection options
• Stable process enabling precise and efficient chemical modifications such as LNA/2'-OMe

RNA Oligo Synthesis Services

Whether sgRNA, crRNA, siRNA, miRNA or ASO, our services can meet your needs.

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