NGS Oligo Synthesis

Next-Generation Sequencing (NGS) is a high-throughput DNA and RNA sequencing technology capable of simultaneously sequencing millions to billions of nucleic acid molecules. Compared to traditional sequencing technologies, NGS offers advantages in speed, cost-effectiveness, and throughput, making it widely applied in genomics, transcriptomics, epigenomics, and other related fields. Throughout NGS workflows, oligonucleotides (oligos) play critical roles in multiple steps. During NGS library preparation, sequence-specific oligo primers are typically employed for PCR amplification to enrich target sequences. Adaptors are short double-stranded DNA fragments and are a key component of NGS library preparation. NGS adaptors contain flow cell binding sequences, the sequencing primer binding sites, and index sequences (barcode). These barcodes are short unique sequences embedded in adaptors for distinguishing different samples when multiple samples are mixed for sequencing. In hybridization-based assays, blocking oligos are utilized to prevent non-specific binding by masking off-target sequences. All oligos involved in NGS workflows are commonly synthetic and can be customized to meet specific sequence needs. NGS applications typically require high purity of oligonucleotides because impurities can interfere with enzyme reactions, reduce library construction efficiency, and even lead to sequencing errors. HPLC or PAGE-purified oligonucleotides are recommended for applications with high purity requirements, such as building complex libraries or using highly sensitive sequencing platforms.

Amerigo Scientific provides NGS oligos characterized by ultra-high purity, site-specific modifications, and minimal cross-contamination, ensuring accuracy in downstream applications. All oligos are synthesized using advanced manufacturing protocols, rigorously purified via PAGE or HPLC, and validated by 100% mass spectrometry to guarantee batch-to-batch consistency. Our solutions address technical challenges such as low base-coupling efficiency, sequence degradation, and operational complexity, offering researchers reliable tools for cutting-edge genomic studies.

Advantages

• ISO 13485 and class 100,000 clean room
• High coupling efficiency
• Low batch-to-batch difference to ensure high homogeneity of multiple target fragments obtained
• Stringent purification standards to ensure high-quality products with less than 0.05% cross contamination rate

NGS Oligo Synthesis Services

In addition to the recommended content, Oligo length and purification methods can also be customized.

Synthesis Workflow

Case

Documents

View and complete our DNA Oligo Synthesis Order Form.