DNA oligonucleotides (oligos) are short, single-stranded DNA molecules, typically consisting of 20 to 30 base pairs. They are usually synthesized as a probe, primer, or as the building blocks for longer DNA strands, and play a crucial role in various molecular biology applications, such as PCR amplification, cloning, sequencing, gene expression analysis, and gene therapy.
Amerigo Scientific provides DNA oligo synthesis services based on cutting-edge technology and strict quality control processes to ensure that customers receive high-quality products with high purity and 100% sequence accuracy. Through the integration of high-quality synthetic raw materials, advanced systems, cutting-edge technology, and professional services, we provide customers with reliable, cost-effective customized DNA oligos to meet the high requirements of research institutions and pharmaceutical or diagnostic development companies.
Advantages
- ISO 13485 certified and Class 100,000 cleanroom
- 100% mass spectrometry with a variety of additional detection options
- Over 200 chemical modifications available
- Flexible synthesis capability from μg to kg to meet the requirements of different specifications
DNA Oligo Synthesis Services
Our DNA oligo synthesis services cater to a wide range of custom requirements, encompassing standard oligo synthesis (5-60 nt), long oligo synthesis (60+ nt), and modified oligo synthesis (5-120 nt). A viarety of purification methods such DSL, OPC, PAGE, and HPLC are available to ensure high product purity. To address specialized customization needs, DNA oligos with modifications such as fluorophores, spacers, and modified bases, or of specific lengths can be synthesized. With guaranteed mass spectrometry verification, we ensure that our customized DNA oligomers are delivered with high precision and high quality, meeting the needs of applications such as molecular biology, genomic research, and drug development.
| Service Type |
Length (nt) |
Purification |
Deliverable |
Application |
| Common Oligos |
5-60 |
DSL/OPC/PAGE/HPLC |
- Tube or Customized
- Lyophilized DNA
- COA Report
|
PCR, DNA sequencing |
| Long Oligos |
60+ |
PAGE/HPLC/Dual PAGE & HPLC |
NGS, genomic research |
| Large-scale Oligos |
Customized |
Customized |
New drug screening, drug production |
| Modified Oligos |
5-120 |
DSL/OPC/PAGE/HPLC/ Dual PAGE & HPLC |
Various molecular biology research |
Available Modification Types
| Modification Types |
Degenerate Bases |
| 5' Mod |
3' Mod |
Double Label Modifications |
Internal Modifications |
Codes |
| 5'Alexa Flour 350 |
3'Alexa Flour 350 |
CY3,BHQ2 |
(dT-Alexa Flour 350) |
B=G,C,T |
| 5'Alexa Flour 405 |
3'Alexa Flour 405 |
CY3,MGB |
(dT-Alexa Flour 405) |
D=A,G,T |
| 5'Alexa Flour 488 |
3'Alexa Flour 488 |
CY5,BHQ2 |
(dT-Alexa Flour 488) |
H=A,C,T |
| 5'Alexa Flour 532 |
3'Alexa Flour 532 |
CY5,BHQ3 |
(dT-Alexa Flour 532) |
K=G,T |
| 5'Alexa Flour 546 |
3'Alexa Flour 546 |
CY5,MGB |
(dT-Alexa Flour 546) |
M=A,C |
| 5'Alexa Flour 555 |
3'Alexa Flour 555 |
CY5.5,BHQ2 |
(dT-Alexa Flour 555) |
N=A,G,C,T |
| 5'Alexa Flour 568 |
3'Alexa Flour 568 |
CY7,BHQ2 |
(dT-Alexa Flour 568) |
R=A,G |
| 5'Alexa Flour 594 |
3'Alexa Flour 594 |
CY7,BHQ3 |
(dT-Alexa Flour 594) |
S=G,C |
| 5'Alexa Fluor 610 |
3'Alexa Fluor 610 |
FAM,BHQ1 |
(dT-Alexa Fluor 610) |
V=A,G,C |
| 5'Alexa Fluor 633 |
3'Alexa Fluor 633 |
FAM,BHQ2 |
(dT-Alexa Fluor 633) |
W=A,T |
| 5'Alexa Flour 647 |
3'Alexa Flour 647 |
FAM,DAB |
(dT-Alexa Flour 647) |
Y=C,T |
| 5'Alexa Flour 680 |
3'Alexa Flour 680 |
FAM,ECL |
(dT-Alexa Flour 680) |
|
| 5'Alexa Fluor 700 |
3'Alexa Fluor 700 |
FAM,MGB |
(dT-Alexa Fluor 700) |
|
| 5'Alexa Fluor 750 |
3'Alexa Fluor 750 |
FAM,TAM |
(dT-Alexa Fluor 750) |
|
| 5'ATTO 425 |
3'ATTO 425 |
HEX,BHQ1 |
(dT-ATTO 425) |
|
| 5'ATTO 550 |
3'ATTO 550 |
HEX,BHQ1 |
(dT-ATTO 550) |
|
| 5'ATTO 565 |
3'ATTO 565 |
HEX,BHQ2 |
(dT-ATTO 565) |
|
| 5'ATTO Rho11 |
3'ATTO Rho11 |
HEX,BHQ2 |
(dT-ATTO Rho11) |
|
| 5'ATTO 594 |
3'ATTO 594 |
HEX,ECL |
(dT-ATTO 594) |
|
| 5'ATTO 655 |
3'ATTO 655 |
HEX,MGB |
(dT-ATTO 655) |
|
| 5'ATTO 700 |
3'ATTO 700 |
HEX,TAM |
(dT-ATTO 700) |
|
| 5'Acrydite |
3'Azide(N3) |
JOE,BHQ1 |
(dT-Azide(N3) |
|
| 5'Azide(N3) |
3'AMCA |
JOE,BHQ2 |
(5F-dU) |
|
| 5'BHQ1 |
3'BHQ0 |
JOE,DAB |
(2-F-rA) |
|
| 5'BHQ2 |
3'BHQ1 |
JOE,MGB |
(2-F-rC) |
|
| 5'BHQ3 |
3'BHQ2 |
JOE,TAM |
(2-F-rG) |
|
| 5'BIO |
3'BHQ3 |
JOE.ECL |
(2-F-rU) |
|
| 5'BIO-TEG |
3'BBQ-650 |
PHO,FAM |
(2'-O-Methyl -A) |
|
| 5'C6-NH2 |
3'BKHFQ (Double Quenching) |
Quasar 670,BHQ3 |
(2'-O-Methyl -C) |
|
| 5'C7-NH2 |
3'BIO |
Quasar570,BHQ2 |
(2'-O-Methyl -G) |
|
| 5'C12-NH2 |
3'BIO-TEG |
ROX,BHQ2 |
(2'-O-Methyl -U) |
|
| 5'CHCH |
3'C3-FAM |
ROX,ECL |
(dT-Digoxin) |
|
| 5'CHO |
3'C6-NH2 |
ROX,MGB |
(dT-DNP) |
|
| 5'Cholesteryl |
3'C7-NH2 |
TAM,BHQ2 |
(dT-BHQ1) |
|
| 5'COOH |
3'CHCH |
TAM,DAB |
(dT-BHQ2) |
|
| 5'CY3 |
3'Cholesteryl |
TAM,ECL |
(dT-BHQ3) |
|
| 5'CY5 |
3'CY3 |
TAM,MGB |
(dT-Biotin) |
|
| 5'CY5-M |
3'CY5 |
TET,BHQ1 |
(dT-Chromeo 494) |
|
| 5'CY5.5 |
3'CY5-M |
TET,BHQ2 |
(dT-CY3) |
|
| 5'CY7 |
3'CY5.5 |
TET,DAB |
(dT-CY5) |
|
| 5'DAB |
3'CY7 |
TET,ECL |
(dT-CY5-M) |
|
| 5'DBCO |
3'DAB |
TET,MGB |
(dT-FAM) |
|
| 5'DIG |
3'DBCO |
TET,TAM |
(dT-FITC) |
|
| 5'ET-ROX |
3'ddC |
TXR,BHQ2 |
(dT-HEX) |
|
| 5'ET-TAMRA |
3'DIG |
TXR,DAB |
(dT-Methylene Blue) |
|
| 5'ET-B (Blue) |
3'ECL |
TXR,MGB |
(dT-Quasar 570) |
|
| 5'ET-G (Green) |
3'Ferrocene |
VIC,BHQ1 |
(dT-Quasar 670) |
|
| 5'ET-Y (Yellow) |
3'HEX |
VIC,BHQ2 |
(dT-Quasar 705) |
|
| 5'ET-R (Red) |
3'InvdT |
VIC,DAB |
(dT-ROX) |
|
| 5'ET-P (Purple) |
3'Joe |
VIC,MGB |
(dT-SF670) |
|
| 5'ET-0 (Orange) |
3'MGB |
VIC,TAM |
(dT-TAM) |
|
| 5'FAM |
3'Methylene Blue |
|
(dT-VIC) |
|
| 5'FITC |
3'PHO |
|
(dT-NH2) |
|
| 5'Ferrocene |
3'QSY7 |
|
(HMC) |
|
| 5'HEX |
3'ROX |
|
(Me-dC) |
|
| 5'HMC |
3'RHO 101 |
|
(PC Linker） |
|
| 5'JOE |
3'SF670 |
|
(C7-NH2) |
|
| 5'Me-DC |
3'-CT+Signal |
|
(dI) |
|
| 5'Methylene Blue |
3'SH |
|
(dU) |
|
| 5'NED |
3'Spacer(C3) |
|
(dSpacer) |
|
| 5'PHO |
3'SS |
|
(Spacer C3) |
|
| 5'Quasar 570 |
3'TAM |
|
(Spacer C6) |
|
| 5'Quasar 670 |
3'TXR |
|
(Spacer 9) |
|
| 5'Quasar 705 |
3'VIC' |
|
(Spacer C12) |
|
| 5'ROX |
|
|
(Spacer 18) |
|
| 5'rApp |
|
|
(SS) |
|
| 5'SF670 |
|
|
(TAO) |
|
| 5'SH |
|
|
(XEN) |
|
| 5'SS |
|
|
-CT+Signal |
|
| 5'TAM |
|
|
LNA-A |
|
| 5'TET |
|
|
LNA-C |
|
| 5'TXR |
|
|
LNA-G |
|
| 5'triple Biotin |
|
|
LNA-T |
|
| 5'VIC |
|
|
(dC-Alexa Flour 350) |
|
| 5'YakYel |
|
|
(dC-Alexa Flour 405) |
|
| |
|
|
(dC-Alexa Flour 488) |
|
| |
|
|
(dC-Alexa Flour 532) |
|
| |
|
|
(dC-Alexa Flour 546) |
|
| |
|
|
(dC-Alexa Flour 555) |
|
Synthesis Workflow
Customization Options
The entire process from synthesis, QC to packaging can be highly customized. Customizable options include, but are not limited to:
- Packaging in 1.5 mL, 15 mL, or 50 mL tubes
- High-performance liquid chromatography (HPLC) analysis
- Quantitative PCR (qPCR) analysis
- OD260/280 measurement
- Specific consumables
- Capillary electrophoresis analysis
- Polyacrylamide gel electrophoresis (PAGE) analysis
- Full-wavelength scanning
- 1 OD sample accompanied by each shipment
- Customized Certificate of Analysis (COA)
Purification Methods
Amerigo Scientific provides a variety of purification methods to ensure high efficiency of purification to obtain high-quality synthetic oligos, including DSL purification, OPC purification, PAGE purification, HPLC purification, and dual PAGE & HPLC purification.
| Method |
Primer length (mer) |
Features |
| 5-8 |
11-40 |
41-60 |
61-90 |
91-140 |
| DSL |
+ |
+ |
+ |
+ |
+ |
It is characterized by rapid and high throughput, and is commonly used in PCR amplification, subcloning, gene synthesis, and Sanger sequencing. |
| OPC |
+ |
++ |
++ |
- |
- |
It is mainly suitable for the purification of oligomers below 60-mer, and its purification purity is better than DSL. |
| PAGE |
- |
+ |
+ |
++ |
++ |
It is commonly used for the purification of oligos above 60-mer and is the best choice for purification of long chain primers. |
| HPLC |
++ |
++ |
++ |
+ |
- |
It is commonly used for the purification of 15 to 60-mer primers and probes with the characteristics of low throughput, high cost and good purity. |
| Dual PAGE & HPLC |
- |
- |
- |
++ |
++ |
It is commonly used for purification of primers and probes with 90-mer or above, and has the characteristics of small throughput, high cost, good purity, and small inter-batch difference. |
++: Recommended; +: Applicable; -: Not applicable
Documents
View and complete our DNA Oligo Synthesis Order Form.
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