Twice Dyed Probes

Real-time polymerase chain reaction (qPCR) is a PCR-based technique that enables reliable detection and measurement of products generated during each cycle of PCR process. This technology allows the quantification of nucleic acids at low template copy numbers with high specificity and accuracy. Among numerous fluorescence-based qPCR assay systems, the highest template specificity comes from those that use double-labeled fluorescent oligonucleotide probes. qPCR based on double-labeled fluorescent probes allows sensitive, specific, and quantitative determination of nucleic acid targets.

Amerigo Scientific offers custom Twice Dyed Probes (TDPs) that are highly sensitive dual-labeled DNA probes. A variety of quenchers and fluorescent dyes are available, and these can be used in a full range of qPCR instrument platforms.

Features of Twice Dyed Probes

• A wide range of quenchers and dyes available
  Including cost-effective alternatives for VIC, NED, and PET dyes
• Fast turnaround time
  Shipped within 5-7 working days
• Ready to go Assays
  Premixed custom primers and TDP probes

Services

Design and synthesize dual-labeled DNA probes with high sensitivity.