Twice Dyed Probes - Amerigo Scientific

Real-time polymerase chain reaction (qPCR) is a PCR-based technique that enables reliable detection and measurement of products generated during each cycle of PCR process. This technology allows the quantification of nucleic acids at low template copy numbers with high specificity and accuracy. Among numerous fluorescence-based qPCR assay systems, the highest template specificity comes from those that use double-labeled fluorescent oligonucleotide probes. qPCR based on double-labeled fluorescent probes allows sensitive, specific, and quantitative determination of nucleic acid targets.

Amerigo Scientific offers custom Twice Dyed Probes (TDPs) that are highly sensitive dual-labeled DNA probes. A variety of quenchers and fluorescent dyes are available, and these can be used in a full range of qPCR instrument platforms.

Features of Twice Dyed Probes

A wide range of quenchers and dyes available
Including cost-effective alternatives for VIC, NED, and PET dyes
Fast turnaround time
Shipped within 5-7 working days
Ready to go Assays
Premixed custom primers and TDP probes

Services

Design and synthesize dual-labeled DNA probes with high sensitivity.

Twice-Dyed Probes	Size	Price
Twice-Dyed Probes with 3' TAMRA
5' FAM + 3' TAMRA	40 nmol / 200 nmol / 1000 nmol	Inquiry
5' HEX + 3' TAMRA	40 nmol / 200 nmol / 1000 nmol	Inquiry
5' Texas Red + 3' TAMRA	40 nmol / 200 nmol / 1000 nmol	Inquiry
5' Cy3 / Cy5 + 3' TAMRA	40 nmol / 200 nmol / 1000 nmol	Inquiry
5' Yakima Yellow + 3' TAMRA	40 nmol / 200 nmol / 1000 nmol	Inquiry
5' VIC replacement + 3' TAMRA	40 nmol / 200 nmol / 1000 nmol	Inquiry
Twice-Dyed Probes with 3' Black Hole Quencher (BBQ, DDQ and Eclipse quencher also available)
5' FAM + 3' BHQ	40 nmol / 200 nmol / 1000 nmol	Inquiry
5' HEX + 3' BHQ	40 nmol / 200 nmol / 1000 nmol	Inquiry
5' Texas Red + 3' BHQ	40 nmol / 200 nmol / 1000 nmol	Inquiry
5' Cy3 + 3' BHQ	40 nmol / 200 nmol / 1000 nmol	Inquiry
5' Cy5 + 3' BHQ	40 nmol / 200 nmol / 1000 nmol	Inquiry
5' JOE + 3' BHQ	40 nmol / 200 nmol / 1000 nmol	Inquiry
5' Dyomic dye + 3' BHQ	40 nmol / 200 nmol / 1000 nmol	Inquiry
5' Yakima Yellow + 3' BHQ	40 nmol / 200 nmol / 1000 nmol	Inquiry
5' LC Replacement + 3' BHQ	40 nmol / 200 nmol / 1000 nmol	Inquiry
5' CAL Fluor dye + 3' BHQ	40 nmol / 200 nmol / 1000 nmol	Inquiry
5' Quasar 705 + 3' BHQ	40 nmol / 200 nmol / 1000 nmol	Inquiry
5' ROX + 3' BHQ	40 nmol / 200 nmol / 1000 nmol	Inquiry
5' Bodipy TMR + 3' BHQ	40 nmol / 200 nmol / 1000 nmol	Inquiry
5' NED replacement + 3' BHQ	40 nmol / 200 nmol / 1000 nmol	Inquiry
5' VIC replacement + 3' BHQ	40 nmol / 200 nmol / 1000 nmol	Inquiry
5' Atto dye + 3' BHQ	40 nmol / 200 nmol / 1000 nmol	Inquiry
5' Alexa Fluor dye + 3' BHQ	40 nmol / 200 nmol / 1000 nmol	Inquiry
Twice-Dyed Probes with internal BHQ and 3'CPR
5' FAM + internal BHQ and 3'CPR	40 nmol / 200 nmol / 1000 nmol	Inquiry
5' Cy5 + internal BHQ and 3'CPR	40 nmol / 200 nmol / 1000 nmol	Inquiry
5' CF Red 590 + internal BHQ and 3'CPR	40 nmol / 200 nmol / 1000 nmol	Inquiry
5' JOE + internal BHQ and 3'CPR	40 nmol / 200 nmol / 1000 nmol	Inquiry