Dual-labeled qPCR probes with sequences less than 20 bases have higher specificity for the target sequence but may have difficulty reaching the melting temperature (Tm) required in the qPCR process. Minor groove binder (MGB) probes are double-labeled probes with conjugated MGB. The MGB moiety increases the Tm of the probe due to its minor groove binding capacity. MGB probes form highly stable duplexes with single-stranded DNA targets, allowing shorter probes to be used for hybridization-based assays. Compare with conventional double-labeled probes, MGB probes have higher Tm and increased specificity, especially when a mismatch occurs in the MGB region of the duplex.
Amerigo Scientific offers customized high-quality MGB probes with short sequences, high stability and specificity.
MGB probe design
Design and synthesize MGB Eclipse® probes with increased stability, specificity, and sensitivity.
MGB Eclipse® Probes | Size | Price |
---|---|---|
3' MGB Eclipse - 5' FAM | 40 nmol / 200 nmol / 1000 nmol | Inquiry |
3' MGB Eclipse - 5' VIC replacem | 40 nmol / 200 nmol / 1000 nmol | Inquiry |
3' MGB Eclipse - 5' HEX | 40 nmol / 200 nmol / 1000 nmol | Inquiry |
3' MGB Eclipse - 5' Atto dye | 40 nmol / 200 nmol / 1000 nmol | Inquiry |
3' MGB Eclipse - 5' Yakima Yellow | 40 nmol / 200 nmol / 1000 nmol | Inquiry |
3' MGB Eclipse - 5' Cy5 | 40 nmol / 200 nmol / 1000 nmol | Inquiry |
3' MGB Eclipse - 5' Texas Red | 40 nmol / 200 nmol / 1000 nmol | Inquiry |
Eclipse is a registered trademark of EliTech Group
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