MAX Probes

Quantitative PCR (qPCR, also known as real-time PCR) is an important method to detect specific DNA sequences in a sample, even trace amounts of DNA sequences. The application of dual-labeled hybridization probes in qPCR has become the most popular of all methods due to their simplicity of operation and the short time required. To obtain reliable results in qPCR experiments, background fluorescence should be reduced to a minimum level. When a long probe (> 25 bases) is necessary, the increased distance between the fluorophore and quencher may reduce the quenching efficiency and increase the background fluorescence. Introducing a second internal quencher in the long probe reduces the background fluorescence and improves the sensitivity and consistency of the qPCR reaction.

Amerigo Scientific offers custom MAX probes that are double-quenched probes with improved quenching efficiency and signal-to-noise ratios. MAX probes consists of a 5' fluorophore dye, a 3' Black Hole Quencher (BHQ™), and an internal quencher incorporated between base residues 9 and 10. This design is particularly suitable for long probe sequences (> 25 bases).

Features of MAX Probes

• Improved quenching efficiency
  The double-quenched design improves quenching efficiency of long probes such as those designed in AT-rich regions.
• Reduced background fluorescence
  Double-quenched probes can be designed to be longer probe sequences with lower background fluorescence, resulting in greater signal- to-noise ratios.
• Increased sensitivity and consistency
  The double quenchers in MAX probes can improve the sensitivity and consistency.

Services

Design and synthesize long qPCR probes with great signal-to-noise ratios.