T-cell Proliferation and Cytokine Analysis
Amerigo Scientific offers cell phenotypic services to facilitate drug discovery and validation. Using our in-house advanced instruments, T cell proliferative responses and multiple cytokines can be accurately analyzed to predict immune responses and evaluate mode of action of compounds. This assay can be used in combination with the Skimune® Sensitisation and Immunotoxicity Testing to determine adverse immune or allergic reactions to drugs and compounds in autologous and allogenic immune settings.
Benefits
- High-throughput multiple-compound and multiple-donor population analysis
- Matched donor/tissue assays
- Human leukocyte antigen (HLA) testing available
- Perfect screening assay for Skimune®
Human In Vitro Skin Explant Test
In an in vitro human skin test for assessing sensitizing potential, the data were presented as the stimulation index (SI) for T-cell proliferation, and the values were the mean of data from at least three independent tests. The results showed that respiratory sensitizers, pre-/pro-haptens and sensitizers induced significant T cell proliferation with SI ≥3. Except phenol (SI = 3.09), the SI values of non-sensitizers were ≤3.
Figure 1. T cell proliferation responses to sensitizers and non-sensitizers. The dashed line indicates a SI value of 3. (An In Vitro human skin test for assessing sensitizing potential. Journal of Applied Toxicology. 2016:36 (5), 669-684.)
Multiplex Cytokine Analysis
Multiplex cytokine analysis of cell culture supernatants could further confirm activation of the human immune system and provide insight into the mode of action of the tested compounds. Th1 and Th2 responses associated with cytotoxicity (such as IFN-γ and TNF-α) or allergic responses (such as IL-4 and IL-13) can be detected by our platform.
Figure 2. Cell culture supernatants analyzed using a proinflammatory panel for IFN-γ, IL-10, IL-12, IL-13, IL-1β, IL-2, IL-4, IL-6 and TNF-α
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