Human blood coagulation Factor XIII-A2, Val34Leu-mutant

- Overview
  - Recombinant human Factor XIII-Val34Leu is a homodimer (a2) composed of two chains held together by non covalent bonds. After activation of the zymogen by Thrombin and Ca2+ to its active form (a*2, Factor XIIIa), Factor XIIIa-Val34Leu catalyzes the formation of covalent bridges (ε-(γ-glutamyl) lysine bonds) between fibrin units to increase the elasticity of the clot network. The resulting cross-linked fibrin is insoluble and resistant to lysis. At position 34 Valine has been replaced by Leucine.Please note that numerotation corresponds to the wild type protein without hexahistidine-tag.
    
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    More Details
    
    Zedira
- Properties
  - Name
    
    Recombinant Fibrin stabilizing factor, protein-glutamine-γ-glutamyltransferase
    
    Source
    
    Insect cells
    
    Type
    
    Recombinant Proteins
    
    Purification
    
    > 95% [by SDS-PAGE]
    
    Formulation
    
    The recombinant human Factor XIII-Val34Leu is lyophilized from 20 mM Tris-HCl pH 7.5, 150 mM NaCl, 1 mM EDTA, 1 mM DTT. Sample contains maltodextrin.
    
    Storage
    
    Storage for several months is possible at ≤ - 20°C. Delivery is possible at ambient temperature
    
    Note
    
    INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.
    
    Molecular Weight
    
    83 kDa (monomer); 166 kDa (homodimer)
    
    Class
    
    Human Factor XIII-A2
    
    Reconstitution
    
    Add the volume of H2O the protein is lyophilized from (see Certificate of Analysis) to the vial of lyophilized powder. Rotate vial gently until solid dissolves. After reconstitution the solution should be stored frozen in working aliquots. Keep cooled on ice for short term storage.
- Applications
  - Application Description
    
    rhFXIIIa-Val34Leu catalyzes acyl transfer reactions from glutamine residues in proteins or peptides to primary amines, e. g. the formation of ε-(γ-glutamyl) lysine bonds between proteins by transferring the acyl group of a peptide-bound glutamine residue to the primary amino group of a peptide-bound lysine residue.
- Reference
  - Heil et al., Thromb. Res. 2013, 131, e214–e22
    Hess et al., PLoS ONE 2012, 7(4): e35690