FXIII-Assay Kit

Catalog Number:
F001
Price:
$1,003
  • Size:
    1 Kit
    Quantity:
    Add to Cart:
      • Overview
        • Transglutaminases are able to also cleave the isopeptide bond. This principle is used to provide easy to handle, robust and precise fluorogenic assays suitable for screening of compound libraries and drug development. Further, it reliably detects levels as low as 5% of the physiological amount of FXIIIa (0.05 IU/ml) in plasma (Oertel et al., Anal. Biochem. 2007, 367:152-8).
          Mechanism: transglutaminase cleaves the isopeptide bond releasing the dark quencher (2,4-dinitrophenyl) linked to the cadaverine spacer. Subsequently, the increase of fluorescence results from the N-terminally attached fluorophore 2-aminobenzoyl (2-Abz).

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      • Properties
        • Formulation
          (1) SUBSTRATE REAGENT: 2 x 20 μL modified peptide dissolved in DMSO
          (2) ACTIVATOR REAGENT: 2 x 100 NIH Units human Thrombin, lyophilized
          (3) BUFFER REAGENT: 2 x TRIS buffer pH 7.5 containing calcium chloride, sodium chloride, poly ethylene glycol (PEG), glycine methyl ester, clot inhibitor peptide, Heparin antagonist (hexadimethrine bromide) and sodium azide, lyophilized
      • Applications
        • Application
          Isopeptidase Fluorogenic Assays
      • Reference
        • Oertel et al., Anal. Biochem. 2007, 367:152-8Sossdorf et al., Crit. Care. 2009,13:R208

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