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Overview
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Fluorescein Labeling Kit-NH2 is mainly used for the preparation of fluorescein-labeled proteins such as IgG for immunostaining and cellular proteins for tracing. Amine-reactive fluorescein, a component of this kit, has succinimidyl groups (NHS) that react with the amino groups on proteins or other molecules. This kit contains all the reagents necessary for labeling, including storage buffer. Each vial of fluorescein can label up to 200 μg of IgG, conjugating about 4 to 6 fluorescein molecules per IgG molecule. Because this kit also includes a buffer exchange system, a sample containing amine base buffer can be labeled. Although membrane filtration sometimes causes IgG aggregation, the buffer system in this kit prevents aggregation during the concentration of IgG or fluorescein-labeled IgG solution. A fluorescein-labeled IgG solution prepared using this kit is stable for more than 2 months at 4°C. The excitation and emission wavelengths of the fluoresceinlabeled IgG are 495 nm and 520 nm, respectively.
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Overview
Fluorescein Labeling Kit-NH2
Fluorescein Labeling Kit-NH2
Catalog Number:
CFA1108131DOJ
Mfr. No.:
LK01-10
Price:
$406
Size:
3 samples
Quantity:
Add to Cart:
.