Biotin Labeling Kit-NH2 - Amerigo Scientific

- Overview
  - Biotin Labeling Kit-NH2 is mainly used for the preparation of biotin-labeled IgG for enzyme immunoassay (EIA). NH2-reactive biotin, a component of this kit, has succinimidyl groups (NHS) that react with amino groups on proteins or other molecules. This kit contains all the reagents necessary for the labeling. The labeling process is very simple. Just add the NH2- reactive Biotin to IgG solution and incubate at 37oC for 10 minutes. An average of 5 to 8 biotin molecules conjugate to each IgG molecule. The number of biotin molecules per protein can be determined by HABA assay. Excess biotin molecules can be removed by a filtration tube.
    
    Please contact us at for specific academic pricing.
- Properties
  - Categories
    
    Biotin labeling
    
    Storage
    
    0-5°C
    
    Shipping
    
    ambient temperature
    
    * For research use only
- Applications
  - Application
    
    FCM; Western blot; Microscopy; Microplate assay
- Reference
  - 1) Y. Kubota, Y. Oike, S. Satoh, Y. Tabata, Y. Niikura, T. Morisada, M. Akao, T. Urano, Y. Ito, T. Miyamoto, N. Nagai, G. Y. Koh, S. Watanabe and T. Suda, "Cooperative Interaction of Angiopoietin-like Proteins 1 and 2 in Zebrafish Vascular Development" , Proc. Natl. Acad. Sci. USA, 2005, 102, 13502.
    
    2) T. Yamabuki, A. Takano, S. Hayama, N. Ishikawa, T. Kato, M. Miyamoto, T. Ito, H. Ito, Y. Miyagi, H. Nakayama, M. Fujita, M. Hosokawa, E. Tsuchiya, N. Kohno, S. Kondo, Y. Nakamura, and Y. Daigo, "Dikkopf-1 as a Novel Serologic and Prognostic Biomarker for Lung and Esophageal Carcinomas", Cancer Res., 2007, 67, 2517.
    
    3) H. Kohara, Y. Omatsu, T. Suhiyama, M. Noda, N. Fujii and T. Nagasawa, "Development of Plasmacytoid Dendritic Cells in Bone Marrow Stromal Cell niches Requires CXCL12-CXCR4 Chemokine Signaling", Blood, 2007, 110, 4153.
    
    4) N. Ishikawa, A. Takano, W. Yasui, K. Inai, H. Nishimura, H. Ito, Y. Miyagi, H. Nakayama, M. Fujita, M. Hosokawa, E. Tsuchiya, N. Kohno, Y. Nakamura and Y. Daigo, "Cancer-testis Antigen Lymphocyte Antigen 6 Complex Locus K is a Serologic Biomarker and a Therapeutic Target for Lung and Esophageal Carcinomas", Cancer Res., 2007, 67, 11601.
    
    5) A. Fukuda, T. Goto, K.N. Kuroishi, K.K. Gunjigake, S. Kataoka, S. Kobayashi and K. Yamaguchi, "Hemokinin-1 competitively inhibits substance P-induced stimulation of osteoclast formation and function", Neuropeptides., 2013, 47, (4), 251.
    
    6) A. Ogawa , M. Sakatsume, X. Wang, Y. Sakamaki, Y. Tsubata, B. Alchi, T. Kuroda, H. Kawachi, I. Narita, F. Shimizu and F. Gejyo, "SM22alpha: the novel phenotype marker of injured glomerular epithelial cells in anti-glomerular basement membrane nephritis", Nephron Exp. Nephrol.., 2007, 106, (3), e77.
    
    7) D. Men, T.T. Zhang, L.W. Hou, J. Zhou, Z.P. Zhang, Y.Y. Shi, J.L. Zhang, Z.Q. Cui, J.Y. Deng, D.B. Wang and X.E. Zhang, "Self-Assembly of Ferritin Nanoparticles into an Enzyme Nanocomposite with Tunable Size for Ultrasensitive Immunoassay", ACS Nano., 2015, 9, (11), 10852.
    
    8) D. Sugahara, Y. Kobayashi, Y. Akimoto, H. Kawakami, "Mouse intestinal niche cells express a distinct α1,2-fucosylated glycan recognized by a lectin from Burkholderia cenocepacia", Glycobiology., 2017, 27, (3), 246.
    
    9) H. Sasaki-Iwaoka, M. Ohori, A. Imasato, K. Taguchi, K. Minoura, T. Saito, K. Kushima, E. Imamura, S. Kubo, S. Furukawa and T. Morokata, "Generation and characterization of a potent fully human monoclonal antibody against the interleukin-23 receptor", Eur. J. Pharmacol.., 2018, 828, 89.
    
    10) K. Kaneshiro, M. Watanabe, K. Terasawa, H. Uchimura, Y. Fukuyama, S. Iwamoto, T.A. Sato, K. Shimizu, G. Tsujimoto and K. Tanaka, "Rapid quantitative profiling of N-glycan by the glycan-labeling method using 3-aminoquinoline/α-cyano-4-hydroxycinnamic acid", Anal. Chem.., 2012, 84, (16), 7146.
    
    11) K.S. Tan, K. Kulkeaw, Y. Nakanishi, D. Sugiyama, "Expression of cytokine and extracellular matrix mRNAs in fetal hepatic stellate cells", Genes Cells., 2017, 22, (9), 836.
    
    12) M. Tahara, S. Ohno, K. Sakai, Y. Ito, H. Fukuhara, K. Komase, M.A. Brindley, P.A. Rota, R.K. Plemper, K. Maenaka and M. Takeda, "The receptor-binding site of the measles virus hemagglutinin protein itself constitutes a conserved neutralizing epitope", J. Virol.., 2013, 87, (6), 3583.
    
    13) M. Takahashi, Y. Ishida, D. Iwaki, K. Kanno, T. Suzuki, Y. Endo, Y. Homma and T. Fujita, "Essential role of Mannose-binding lectin-associated serine protease-1 in activation of the complement factor D", J. Exp. Med.., 2010, 207, (1), 29.
    
    14) N. Hosen, Y. Matsunaga, K. Hasegawa, H. Matsuno, Y. Nakamura, M. Makita, K. Watanabe, M. Yoshida, K. Satoh, S. Morimoto, F. Fujiki, H. Nakajima, J. Nakata, S. Nishida, A. Tsuboi, Y. Oka, M. Manabe, H. Ichihara, Y. Aoyama, A. Mugitani , T. Nakao, M. Hino, R. Uchibori, K. Ozawa, Y. Baba, S. Terakura, N. Wada, E. Morii, J. Nishimura, K. Takeda, Y. Oji, H. Sugiyama, J. Takagi and A. Kumanogoh, "The activated conformation of integrin β7 is a novel multiple myeloma-specific target for CAR T cell therapy", Nat. Med.., 2017, 23, (12), 1436.
    
    15) R. Nishino, A. Takano, H. Oshita, N. Ishikawa, H. Akiyama, H. Ito, H. Nakayama, Y. Miyagi, E. Tsuchiya, N. Kohno, Y. Nakamura and Y. Daigo, "Identification of Epstein-Barr virus–induced gene 3 as a novel serum and tissue biomarker and a therapeutic target for lung cancer", Clin. Cancer Res.., 2011, 17, (19), 6272.
    
    16) T. Hiono, A. Matsuda, T. Wagatsuma, M. Okamatsu, Y. Sakoda and A. Kuno, "Lectin microarray analyses reveal host cell-specific glycan profiles of the hemagglutinins of influenza A viruses", Virology., 2019, 527, 132.
    
    17) T. Oshima, S. Sato, J. Kato, Y. Ito, T. Watanabe, I. Tsuji, A. Hori, T. Kurokawa and T. Kokubo, "Nectin-2 is a potential target for antibody therapy of breast and ovarian cancers", Mol. Cancer., 2013, 12, (60), .
    
    18) T. Yoshida, N. Shiraki, H. Baba, M. Goto, S. Fujiwara, K. Kume and S. Kume, "Expression patterns of epiplakin1 in pancreas, pancreatic cancer and regenerating pancreas", Genes Cells., 2008, 13, (7), 667.
    
    19) X. Piao, T. Ozawa, H. Hamana, K. Shitaoka, A. Jin, H. Kishi and A. Muraguchi, "TRAIL-receptor 1 IgM antibodies strongly induce apoptosis in human cancer cells in vitro and in vivo", Oncoimmunology., 2016, 5, (5), e1131380.
    
    20) Y. Shimazaki, Y. Kohno, "Successive analysis of antigen trapping and enzymatic digestion on membrane-immobilized avidin", Anal. Biochem.., 2012, 422, (1), 55.
    
    21) T. Kaya, S. Nagatoishi, K. Nagae, Y. Nakamura and K. Tsumoto , "Highly sensitive biomolecular interaction detection method using optical bound/free separation with grating-coupled surface plasmon field-enhanced fluorescence spectroscopy (GC-SPFS).", PLoS ONE., 2019, 14, (8), DOI:10.1371/journal.pone.0220578.