HiLyte Fluor 555 Labeling Kit-NH2

- Overview
  - HiLyte Fluor* 555 Labeling Kit-NH2 is used mainly for the preparation of red fluorescence-labeled proteins, such as IgG, for immunostaining, and cellular proteins for tracing. NH2-reactive HiLyteFluor 555, a component of this kit, has a succinimidyl group (NHS) that reacts with amino groups on proteins or other molecules. This kit contains all the reagents necessary for labeling. Each tube of HiLyte Fluor 555 can label up to 200 μg of IgG, conjugating about 4 to 6 HiLyte Fluor 555 molecules per IgG molecule. The labeling process is simple-add the NH2-reactive HiLyte Fluor 555 to IgG solution on a membrane and incubate at 37°C for 10 minutes. The excess HiLyte Fluor 555 molecules can be removed by a filtration tube. The excitation and emission wavelengths of the HiLyte Fluor 555-labeled IgG are 555 nm and 570 nm, respectively.* HiLyte Fluor is a trademark of AnaSpec, Inc.
    
    Please contact us at for specific academic pricing.
- Properties
  - Categories
    
    Fluorophore labeling
    
    Storage
    
    0-5°C
    
    Shipping
    
    ambient temperature
    
    * For research use only
- Applications
  - Application
    
    FCM; Microscopy
- Reference
  - 1) A. Nagao, K. Sato, K.M. Nishida, H. Siomi, M.C. Siomi, "Gender-specific hierarchy in nuage localization of PIWI-interacting RNA factors in Drosophila", Front Genet., 2011, 2, 55.
    
    2) H. Hoshino, G. Shioi, S. Aizawa, "AVE protein expression and visceral endoderm cell behavior during anterior–posterior axis formation in mouse embryos: Asymmetry in OTX2 and DKK1 expression", Dev. Biol.., 2015, 402, (2), 175.
    
    3) T. Kaneko, T. Minohara, S. Shima, K. Yoshida, A. Fukuda, N. Iwamori, T. Inai and H. Iida, "A membrane protein, TMCO5A, has a close relationship with manchette microtubules in rat spermatids during spermiogenesis", Mol. Reprod. Dev.., 2019, 86, (3), 330.
    
    4) Y. Nakashima, T. Mima, M. Yashiro, T. Sonou, M. Ohya, A. Masumoto, S. Yamanaka, D. Koreeda, K. Tatsuta, Y. Hanba, M. Moribata, S. Negi, T. Shigematsu, "Expression and localization of fibroblast growth factor (FGF) 23 and Klotho in the spleen: its physiological and functional implications", Growth Factors ., 2016, 34, (5-6), 196.
    
    5) Z. Zhang, K. Kakutani, K. Maeno, T. Takada, T. Yurube, M. Doita, M. Kurosaka and K. Nishida, "Expression of silent mating type information regulator 2 homolog 1 and its role in human intervertebral disc cell homeostasis", Arthritis Res. Ther.., 2011, 13, (6), R200.